A regulative two-component system previously identified in Pseudoalteromonas haloplanktis TAC125 was used to construct a cold inducible expression system that is under the control of l-malate. Performances of the inducible system were tested for both psychrophilic and mesophilic protein production using two “difficult” proteins as control. The results obtained demonstrated that both psychrophilic -galactosidase and yeast -glucosidase are produced in a fully soluble and catalytically competent form. Optimal conditions for protein production, including growth temperature, growth medium and l-malate concentration were also investigated. Under optimized conditions yields of 620 and 27 mg/l were obtained for -galactosidase and -glucosidase, respectively.
An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125 / Papa, Rosanna; V., Rippa; G., Sannia; G., Marino; A., Duilio. - In: JOURNAL OF BIOTECHNOLOGY. - ISSN 0168-1656. - STAMPA. - 2:126(2007), pp. 199-210. [doi:10.1016/j.jbiotec.2006.07.003]
An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125
PAPA, Rosanna;
2007
Abstract
A regulative two-component system previously identified in Pseudoalteromonas haloplanktis TAC125 was used to construct a cold inducible expression system that is under the control of l-malate. Performances of the inducible system were tested for both psychrophilic and mesophilic protein production using two “difficult” proteins as control. The results obtained demonstrated that both psychrophilic -galactosidase and yeast -glucosidase are produced in a fully soluble and catalytically competent form. Optimal conditions for protein production, including growth temperature, growth medium and l-malate concentration were also investigated. Under optimized conditions yields of 620 and 27 mg/l were obtained for -galactosidase and -glucosidase, respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.