Four commercial enzyme preparations containing cell-wall degrading enzyme activities were tested for their ability to facilitate lycopene extraction from tomato paste. At 25 degrees C, up to 75.6% of the lycopene present in the tomato product was extracted by a two-stage procedure that consisted of a preliminary 5 hour enzyme incubation followed by a 3 hour solvent (hexane or ethyl acetate) extraction. Increasing the duration of the enzymatic treatment to 12-18 h allowed the recovery of 85-90% of total lycopene. The highest extraction yields were achieved by using enzyme preparations' with polygalacturonase and pectin methylesterase in addition to pectin lyase or cellulase activities.
MILD ENZYMATIC METHOD FOR THE EXTRACTION OF LYCOPENE FROM TOMATO PASTE / Zuorro, Antonio; Lavecchia, Roberto. - In: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT. - ISSN 1310-2818. - STAMPA. - 24:2(2010), pp. 1854-1857. [10.2478/v10133-010-0028-0]
MILD ENZYMATIC METHOD FOR THE EXTRACTION OF LYCOPENE FROM TOMATO PASTE
ZUORRO, ANTONIO;LAVECCHIA, Roberto
2010
Abstract
Four commercial enzyme preparations containing cell-wall degrading enzyme activities were tested for their ability to facilitate lycopene extraction from tomato paste. At 25 degrees C, up to 75.6% of the lycopene present in the tomato product was extracted by a two-stage procedure that consisted of a preliminary 5 hour enzyme incubation followed by a 3 hour solvent (hexane or ethyl acetate) extraction. Increasing the duration of the enzymatic treatment to 12-18 h allowed the recovery of 85-90% of total lycopene. The highest extraction yields were achieved by using enzyme preparations' with polygalacturonase and pectin methylesterase in addition to pectin lyase or cellulase activities.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
