The potential use of the enzyme catalase to oxygenate blood was investigated. A microcalorimetric technique was used to study the kinetics of oxygen generation in human blood serum at 25 and 37 degrees C. Experiments carried out in serum and in phosphate buffer provided the apparent rate constants for the enzymatic and non-enzymatic oxygen-generation reactions. A simplified mathematical model was then developed to analyse the behavior of a hollow-fiber membrane oxygenator with catalase covalently bound to the membrane surface. The model was used to simulate an extracorporeal oxygenation treatment and outline differences with current devices. (C) 1999 Elsevier Science Ltd. All rights reserved.
An enzymatic membrane reactor for extracorporeal blood oxygenation / Federico, Cioci; Lavecchia, Roberto; Pierfrancesco, Mazzocchi. - In: CHEMICAL ENGINEERING SCIENCE. - ISSN 0009-2509. - 54:15-16(1999), pp. 3217-3223. (Intervento presentato al convegno 15th International Symposium on Chemical Reaction Engineering (ISCRE 15) tenutosi a NEWPORT BEACH, CALIFORNIA nel SEP 13-16, 1998) [10.1016/s0009-2509(98)00348-0].
An enzymatic membrane reactor for extracorporeal blood oxygenation
LAVECCHIA, Roberto;
1999
Abstract
The potential use of the enzyme catalase to oxygenate blood was investigated. A microcalorimetric technique was used to study the kinetics of oxygen generation in human blood serum at 25 and 37 degrees C. Experiments carried out in serum and in phosphate buffer provided the apparent rate constants for the enzymatic and non-enzymatic oxygen-generation reactions. A simplified mathematical model was then developed to analyse the behavior of a hollow-fiber membrane oxygenator with catalase covalently bound to the membrane surface. The model was used to simulate an extracorporeal oxygenation treatment and outline differences with current devices. (C) 1999 Elsevier Science Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
