On their path to draining lymph nodes, epidermal Langerhans cells traverse collagen-dense connective tissue before reaching lymphatic vessels. The matricellular protein SPARC (secreted protein, acidic and rich in cysteine), which is induced during inflammation and tissue repair, organizes collagen deposition in tissue stroma. We analyzed Langerhans cell and dendritic-cell migration and its impact on T-cell priming in SPARC-null (SPARC(-/-)) and SPARC-sufficient (SPARC(+/+)) mice. Although the same number of Langerhans cells populate the ear skin of SPARC(-/-) and SPARC(+/+) mice, more Langerhans cells were found in the lymph nodes draining antigen-sensitized ears of SPARC(-/-) mice and significantly more Langerhans cells migrated from null-mice-derived ear skin explants. Such favored Langerhans cell migration is due to the host environment, as demonstrated by SPARC(+/+)>SPARC(-/-) and reciprocal chimeras, and have a profound influence on T-cell priming. Contact-, delayed type-hypersensitivity and naive T-cell receptor-transgenic T-cell priming, together indicate that the lack of SPARC in the environment accelerates the onset of T-cell priming by hastening Langerhans cell/dendritic-cell migration.

Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice / S., Sangalletti; L., Gioiosa; C., Guiducci; M., Rescigno; Stoppacciaro, Antonella; C., Chiodoni; M. P., Colombo. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 118:16(2005), pp. 3685-3694. [10.1242/jcs.02474]

Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice

STOPPACCIARO, ANTONELLA;
2005

Abstract

On their path to draining lymph nodes, epidermal Langerhans cells traverse collagen-dense connective tissue before reaching lymphatic vessels. The matricellular protein SPARC (secreted protein, acidic and rich in cysteine), which is induced during inflammation and tissue repair, organizes collagen deposition in tissue stroma. We analyzed Langerhans cell and dendritic-cell migration and its impact on T-cell priming in SPARC-null (SPARC(-/-)) and SPARC-sufficient (SPARC(+/+)) mice. Although the same number of Langerhans cells populate the ear skin of SPARC(-/-) and SPARC(+/+) mice, more Langerhans cells were found in the lymph nodes draining antigen-sensitized ears of SPARC(-/-) mice and significantly more Langerhans cells migrated from null-mice-derived ear skin explants. Such favored Langerhans cell migration is due to the host environment, as demonstrated by SPARC(+/+)>SPARC(-/-) and reciprocal chimeras, and have a profound influence on T-cell priming. Contact-, delayed type-hypersensitivity and naive T-cell receptor-transgenic T-cell priming, together indicate that the lack of SPARC in the environment accelerates the onset of T-cell priming by hastening Langerhans cell/dendritic-cell migration.
dc migration; sparc; t-cell priming
01 Pubblicazione su rivista::01a Articolo in rivista
Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice / S., Sangalletti; L., Gioiosa; C., Guiducci; M., Rescigno; Stoppacciaro, Antonella; C., Chiodoni; M. P., Colombo. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 118:16(2005), pp. 3685-3694. [10.1242/jcs.02474]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/67154
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