The passage of DNA through a nanopore can be effectively decomposed into two distinct phases, docking and actual translocation. In experiments each phase is characterized by a distinct current signature which allows the discrimination of the two events. However, at low voltages a clear distinction of the two phases is lost. By using numerical simulations we clarify how the current signature associated to the docking events depends on the applied voltage. The simulations show that at small voltage the DNA globule enhances the pore conductance due to an enrichment of charge carriers. At high voltage, the globule drains substantial charge carriers from the pore region, thereby reducing the overall conductance. The results provide a new interpretation to the experimental data on conductance and show how docking interferes with the translocation signal, of potential interest for sequencing applications.
Modulation of current through a nanopore induced by a charged globule: Implications for DNA-docking / Chinappi, Mauro; Casciola, Carlo Massimo; Cecconi, Fabio; U. M. B., Marconi; Melchionna, Simone. - In: EUROPHYSICS LETTERS. - ISSN 0295-5075. - STAMPA. - 108 (4):(2014), p. 46002. [10.1209/0295-5075/108/46002]
Modulation of current through a nanopore induced by a charged globule: Implications for DNA-docking
CHINAPPI, MAURO;CASCIOLA, Carlo Massimo;CECCONI, FABIO;MELCHIONNA, SIMONE
2014
Abstract
The passage of DNA through a nanopore can be effectively decomposed into two distinct phases, docking and actual translocation. In experiments each phase is characterized by a distinct current signature which allows the discrimination of the two events. However, at low voltages a clear distinction of the two phases is lost. By using numerical simulations we clarify how the current signature associated to the docking events depends on the applied voltage. The simulations show that at small voltage the DNA globule enhances the pore conductance due to an enrichment of charge carriers. At high voltage, the globule drains substantial charge carriers from the pore region, thereby reducing the overall conductance. The results provide a new interpretation to the experimental data on conductance and show how docking interferes with the translocation signal, of potential interest for sequencing applications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.