PURPOSE: Based on a genome-wide association study of testicular dysgenesis syndrome showing a possible association with TGFBR3, we analyzed data from a larger, phenotypically restricted cryptorchidism population for potential replication of this signal. MATERIALS AND METHODS: We excluded samples based on strict quality control criteria, leaving 844 cases and 2,718 controls of European ancestry that were analyzed in 2 separate groups based on genotyping platform (ie Illumina® HumanHap550, version 1 or 3, or Human610-Quad, version 1 BeadChip in group 1 and Human OmniExpress 12, version 1 BeadChip platform in group 2). Analyses included genotype imputation at the TGFBR3 locus, association analysis of imputed data with correction for population substructure, subsequent meta-analysis of data for groups 1 and 2, and selective genotyping of independent cases (330) and controls (324) for replication. We also measured Tgfbr3 mRNA levels and performed TGFBR3/betaglycan immunostaining in rat fetal gubernaculum. RESULTS: We identified suggestive (p ≤ 1× 10(-4)) association of markers in/near TGFBR3, including rs9661103 (OR 1.40; 95% CI 1.20, 1.64; p = 2.71 × 10(-5)) and rs10782968 (OR 1.58; 95% CI 1.26, 1.98; p = 9.36 × 10(-5)) in groups 1 and 2, respectively. In subgroup analyses we observed strongest association of rs17576372 (OR 1.42; 95% CI 1.24, 1.60; p = 1.67 × 10(-4)) with proximal and rs11165059 (OR 1.32; 95% CI 1.15, 1.38; p = 9.42 × 10(-4)) with distal testis position, signals in strong linkage disequilibrium with rs9661103 and rs10782968, respectively. Association of the prior genome-wide association study signal (rs12082710) was marginal (OR 1.13; 95% CI 0.99, 1.28; p = 0.09 for group 1), and we were unable to replicate signals in our independent cohort. Tgfbr3/betaglycan was differentially expressed in wild-type and cryptorchid rat fetal gubernaculum. CONCLUSIONS: These data suggest complex or phenotype specific association of cryptorchidism with TGFBR3 and the gubernaculum as a potential target of TGFβ signaling.
Phenotype-specific association of the TGFBR3 locus with nonsyndromic cryptorchidism / Barthold, J; Wang, Y; Olivant Fisher, A; Kolon, T; Nordenskjöld, A; Figueroa, E; BaniHani, A; Hagerty, J; Gonzalez, R; Noh, P; Chiavacci, R; Harden, K; Abrams, D; Mateson, A; Robbins, A; Li, J; Akins, R; Hakonarson, H; Devoto, Marcella. - In: THE JOURNAL OF UROLOGY. - ISSN 0022-5347. - 193(2015), pp. 1637-1645.
|Titolo:||Phenotype-specific association of the TGFBR3 locus with nonsyndromic cryptorchidism|
|Data di pubblicazione:||2015|
|Citazione:||Phenotype-specific association of the TGFBR3 locus with nonsyndromic cryptorchidism / Barthold, J; Wang, Y; Olivant Fisher, A; Kolon, T; Nordenskjöld, A; Figueroa, E; BaniHani, A; Hagerty, J; Gonzalez, R; Noh, P; Chiavacci, R; Harden, K; Abrams, D; Mateson, A; Robbins, A; Li, J; Akins, R; Hakonarson, H; Devoto, Marcella. - In: THE JOURNAL OF UROLOGY. - ISSN 0022-5347. - 193(2015), pp. 1637-1645.|
|Appartiene alla tipologia:||01a Articolo in rivista|