In the airways Cl− ions are secreted by the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) channel that also limits Na+ absorption by partially repressing the Epithelial Na+ Channel (ENaC). The mechanisms regulating the transcription of the 3 ENaC genes (SCNN1A, SCNN1B and SCNN1G) are not fully elucidated and only sporadic experimental evidence exists on their possible epigenetic regulation. We studied the expression of the 3 ENaC genes and the DNA methylation levels of their 5′-flanking regions in H441, MCF10A, 16HBE, CFBE and HACAT in vitro cell lines, as well as in nasal brushing, granulocytes, lymphocytes and monocytes of subjects with no Cystic Fibrosis (CF).
Expression and DNA methylation of ENaC genes / Lucarelli, Marco; Ceci, Fabrizio; Truglio, Gessica; Pierandei, S.; Carofiglio, F.; Ascenzioni, F.; Quattrucci, Serena; Strom, Roberto. - ELETTRONICO. - 9:(2010), pp. S93-S93. (Intervento presentato al convegno 33rd European Cystic Fibrosis Conference tenutosi a Valencia, Spagna nel 16-19 giungo 2010).
Expression and DNA methylation of ENaC genes
LUCARELLI, Marco;CECI, Fabrizio;TRUGLIO, GESSICA;F. Ascenzioni;QUATTRUCCI, Serena;STROM, Roberto
2010
Abstract
In the airways Cl− ions are secreted by the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) channel that also limits Na+ absorption by partially repressing the Epithelial Na+ Channel (ENaC). The mechanisms regulating the transcription of the 3 ENaC genes (SCNN1A, SCNN1B and SCNN1G) are not fully elucidated and only sporadic experimental evidence exists on their possible epigenetic regulation. We studied the expression of the 3 ENaC genes and the DNA methylation levels of their 5′-flanking regions in H441, MCF10A, 16HBE, CFBE and HACAT in vitro cell lines, as well as in nasal brushing, granulocytes, lymphocytes and monocytes of subjects with no Cystic Fibrosis (CF).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
