The expression and effects of the plant oncogene rolB during in vitro flower and root morphogenesis were investigated. Thin cell layers (TCL) were excised from floral pedicels and from stems of transgenic tobacco plants containing either rolB and the promoter of rolB fused to the GUS reporter gene (BpB-GUS plants) or only this latter construct (pB-GUS plants). Organogenesis and GUS activity were monitored in pedicel and stem TCLs for 30 days under conditions of floral or root neoformation. Both flowering and rhizogenesis are substantially enhanced and more precocious in rolB-transformed explants than in TCLs from pB-GUS and control plants. Most interestingly, both in flower and in root neoformation, meristemoids are much more numerous and appear sooner in rolB-transformed tissues. Thus, the effect of rolB does not seem to be specifically the induction of rooting, but, rather, the promotion of meristem formation, regardless of subsequent differentiation. In addition, we provide evidence that the presence of the gene product of rolB does not alter the pattern and timing of expression of the gene.
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|Titolo:||THE PLANT ONCOGENE ROLB STIMULATES THE FORMATION OF FLOWER AND ROOT MERISTEMOIDS IN TOBACCO THIN CELL-LAYERS|
|Data di pubblicazione:||1994|
|Citazione:||THE PLANT ONCOGENE ROLB STIMULATES THE FORMATION OF FLOWER AND ROOT MERISTEMOIDS IN TOBACCO THIN CELL-LAYERS / Altamura, Maria Maddalena; Capitani, Francesca; L., Gazza; I., Capone; Costantino, Paolo. - In: NEW PHYTOLOGIST. - ISSN 0028-646X. - STAMPA. - 126:2(1994), pp. 283-293.|
|Appartiene alla tipologia:||01a Articolo in rivista|