A lab-on-chip for the diagnosis of celiac disease relying on the monitoring of patient-specific immune response to gliadin fractions has been developed. The detection is based on a chemiluminescent immunoenzymatic reaction that ensures high specificity and sensitivity. The chemiluminescent signal is monitored by hydrogenated amorphous silicon photosensors, fabricated on the same glass substrate hosting the biochemical recognition. The main challenge of the work has been the identification of the materials and the setup of the entire process that permitted the reliable fabrication of the device. Experiments performed with serum samples of rabbit immunized towards an epitope show a good specificity of the proposed technique, proving the feasibility of an integrated device for the patient-specific profiling. © 2014 Springer International Publishing Switzerland.
On-chip diagnosis of celiac disease by an amorphous silicon chemiluminescence detector / Caputo, Domenico; DE CESARE, Giampiero; Scipinotti, Riccardo; N., Stasio; Costantini, Francesca; Manetti, Cesare; Nascetti, Augusto. - STAMPA. - 268 LNEE:(2014), pp. 183-187. (Intervento presentato al convegno 17th National Conference on Sensors and Microsystems tenutosi a Brescia nel 5 February 2013 through 7 February 2013) [10.1007/978-3-319-00684-0-35].
On-chip diagnosis of celiac disease by an amorphous silicon chemiluminescence detector
CAPUTO, Domenico;DE CESARE, Giampiero;SCIPINOTTI, RICCARDO;COSTANTINI, FRANCESCA;MANETTI, Cesare;NASCETTI, Augusto
2014
Abstract
A lab-on-chip for the diagnosis of celiac disease relying on the monitoring of patient-specific immune response to gliadin fractions has been developed. The detection is based on a chemiluminescent immunoenzymatic reaction that ensures high specificity and sensitivity. The chemiluminescent signal is monitored by hydrogenated amorphous silicon photosensors, fabricated on the same glass substrate hosting the biochemical recognition. The main challenge of the work has been the identification of the materials and the setup of the entire process that permitted the reliable fabrication of the device. Experiments performed with serum samples of rabbit immunized towards an epitope show a good specificity of the proposed technique, proving the feasibility of an integrated device for the patient-specific profiling. © 2014 Springer International Publishing Switzerland.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
