Circadian rhythms are highly conserved time tracking systems regulating important biological processes at both systemic and cellular levels. The present study was aimed to identify proteins and biological functions circadian regulated in human keratinocytes. HaCaT keratinocytes were entrained by temperature cycles, and a proteomic study was performed on cell fractions isolated under free running conditions at constant temperature. Bioinformatics analysis revealed that molecular clock entrainment was associated with changes in molecular components regulating cell proliferation, energy metabolism, transcription, translation and redox balance. Nuclear levels of the antioxidant enzyme Peroxiredoxin 2 (PRDX2) were found to oscillate rhythmically over two entire 24 h long cycles. Donwregulation of PRDX2 resulted in upregulation of the mitochondrion-specific Peroxiredoxin 3 (PRDX3), all other members of the Peroxiredoxin family remained unaltered. Furthermore, PRDX2 knockdown increased intracellular levels of reactive oxygen species (ROS) and impaired cell cycle progression and proliferation. HaCaT cells transduced with a scramble shRNA were used as control. Our work is the first to show that nuclear levels of PRDX2 display circadian oscillation participating in the regulation of human keratinocytes redox balance. © 2014 Elsevier Ltd.

Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes / Avitabile, Daniele; Ranieri, Danilo; Nicolussi, Arianna; D'Inzeo, Sonia; Capriotti, ANNA LAURA; Genovese, Licia; Proietti, Sara; Cucina, Alessandra; Coppa, Anna; Samperi, Roberto; Bizzarri, Mariano; Lagana', Aldo; Torrisi, Maria Rosaria. - In: THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY. - ISSN 1357-2725. - 53:(2014), pp. 24-34. [10.1016/j.biocel.2014.04.024]

Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes

AVITABILE, DANIELE;RANIERI, DANILO;NICOLUSSI, Arianna;D'INZEO, SONIA;CAPRIOTTI, ANNA LAURA;GENOVESE, Licia;PROIETTI, SARA;CUCINA, Alessandra;COPPA, Anna;SAMPERI, Roberto;BIZZARRI, Mariano;LAGANA', Aldo;TORRISI, Maria Rosaria
2014

Abstract

Circadian rhythms are highly conserved time tracking systems regulating important biological processes at both systemic and cellular levels. The present study was aimed to identify proteins and biological functions circadian regulated in human keratinocytes. HaCaT keratinocytes were entrained by temperature cycles, and a proteomic study was performed on cell fractions isolated under free running conditions at constant temperature. Bioinformatics analysis revealed that molecular clock entrainment was associated with changes in molecular components regulating cell proliferation, energy metabolism, transcription, translation and redox balance. Nuclear levels of the antioxidant enzyme Peroxiredoxin 2 (PRDX2) were found to oscillate rhythmically over two entire 24 h long cycles. Donwregulation of PRDX2 resulted in upregulation of the mitochondrion-specific Peroxiredoxin 3 (PRDX3), all other members of the Peroxiredoxin family remained unaltered. Furthermore, PRDX2 knockdown increased intracellular levels of reactive oxygen species (ROS) and impaired cell cycle progression and proliferation. HaCaT cells transduced with a scramble shRNA were used as control. Our work is the first to show that nuclear levels of PRDX2 display circadian oscillation participating in the regulation of human keratinocytes redox balance. © 2014 Elsevier Ltd.
2014
circadian rhythm; proteomic analysis; nano-lc-ms/ms; peroxiredoxin 2; metabolic clock; hacat keratinocytes
01 Pubblicazione su rivista::01a Articolo in rivista
Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes / Avitabile, Daniele; Ranieri, Danilo; Nicolussi, Arianna; D'Inzeo, Sonia; Capriotti, ANNA LAURA; Genovese, Licia; Proietti, Sara; Cucina, Alessandra; Coppa, Anna; Samperi, Roberto; Bizzarri, Mariano; Lagana', Aldo; Torrisi, Maria Rosaria. - In: THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY. - ISSN 1357-2725. - 53:(2014), pp. 24-34. [10.1016/j.biocel.2014.04.024]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/563320
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