There is a need for chiral stationary phases (CSPs) designed for high performance liquid chromatography (HPLC) to switch to enantioselective applications using ultrahigh-performance liquid chromatography (UHPLC). Although important goals have been achieved to rapidly separate achiral compounds, enantioselective LC remains solidly attached to 3-μm and 5-μm totally porous particles and pressure values in the HPLC domain. This article describes strategies aimed at immobilizing or coating well established chiral selectors onto sub-2-μm silica particles, and aims to illustrate the potential of enantioselective UHPLC (eUHPLC) in terms of high speed, throughput and resolution.
The evolution of chiral stationary phases from HPLC to UHPLC / D'Acquarica, Ilaria; Kotoni, Dorina; Ciogli, Alessia; Pierini, Marco; J., Kocergin; H., Ritchie; Villani, Claudio; Gasparrini, Francesco. - In: LC GC EUROPE. - ISSN 1471-6577. - STAMPA. - 27(3):(2014), pp. 128-137.
The evolution of chiral stationary phases from HPLC to UHPLC.
D'ACQUARICA, Ilaria;KOTONI, DORINA;CIOGLI, Alessia;PIERINI, MARCO;VILLANI, Claudio;GASPARRINI, Francesco
2014
Abstract
There is a need for chiral stationary phases (CSPs) designed for high performance liquid chromatography (HPLC) to switch to enantioselective applications using ultrahigh-performance liquid chromatography (UHPLC). Although important goals have been achieved to rapidly separate achiral compounds, enantioselective LC remains solidly attached to 3-μm and 5-μm totally porous particles and pressure values in the HPLC domain. This article describes strategies aimed at immobilizing or coating well established chiral selectors onto sub-2-μm silica particles, and aims to illustrate the potential of enantioselective UHPLC (eUHPLC) in terms of high speed, throughput and resolution.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
