Objectives:The aim of the study was to determine whether the release by macrophages of matrix metalloproteinase (MMP)-12 and vascular endothelial growth factor (VEGF) - leading to inflammation, matrix degradation and neoangiogenesis - represents an effective pathway that underlies aortic wall remodeling in Stanford type A acute aortic dissection (AAD). Methods: Twenty-one consecutive patients with no genetic predisposition, with Stanford type A AAD were selected. In each patient, the levels of serum VEGF, MMP-12, serum interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1 were evaluated using enzyme-linked immunosorbent assay. Ascending aortic specimens were collected for immunohistochemical identification of any presence of inflammatory infiltrate, VEGF and CD31 expression. Results: A significant increase in serum VEGF (p = 0.044), MMP-12 (p = 0.007), IL-6 (p = 0.0001), IL-8 (p = 0.0001) and MCP-1 (p = 0.0001) levels was observed in the AAD group compared to the control group. Furthermore, all AAD samples were positive for VEGF in the tunica media and showed vessel growth and immune-inflammatory infiltrate. A large number of cases (62.79%) showed inflammation at the edge of the dissection and approximately half (51.42%) showed neovessels growing at the edge of the dissection. Conclusions:The results suggest that VEGF-mediated angiogenesis and matrix degradation play a role in AAD. Finally, we believe that MMP-12 should be considered a marker of AAD. (C) 2013 S. Karger AG, Basel

The multitasking role of macrophages in stanford type a acute aortic dissection / DEL PORTO, Flavia; DI GIOIA, Cira Rosaria Tiziana; Tritapepe, Luigi; Ferri, Livia; Leopizzi, Martina; Nofroni, Italo; Vincenzo De, Santis; DELLA ROCCA, Carlo; Mitterhofer, Anna Paola; Bruno, Guglielmo; Taurino, Maurizio; Maria, Proietta. - In: CARDIOLOGY. - ISSN 0008-6312. - 127:2(2014), pp. 123-129. [10.1159/000355253]

The multitasking role of macrophages in stanford type a acute aortic dissection

DEL PORTO, Flavia;DI GIOIA, Cira Rosaria Tiziana;TRITAPEPE, Luigi;FERRI, LIVIA;LEOPIZZI, MARTINA;NOFRONI, Italo;DELLA ROCCA, Carlo;MITTERHOFER, Anna Paola;BRUNO, Guglielmo;TAURINO, Maurizio;
2014

Abstract

Objectives:The aim of the study was to determine whether the release by macrophages of matrix metalloproteinase (MMP)-12 and vascular endothelial growth factor (VEGF) - leading to inflammation, matrix degradation and neoangiogenesis - represents an effective pathway that underlies aortic wall remodeling in Stanford type A acute aortic dissection (AAD). Methods: Twenty-one consecutive patients with no genetic predisposition, with Stanford type A AAD were selected. In each patient, the levels of serum VEGF, MMP-12, serum interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1 were evaluated using enzyme-linked immunosorbent assay. Ascending aortic specimens were collected for immunohistochemical identification of any presence of inflammatory infiltrate, VEGF and CD31 expression. Results: A significant increase in serum VEGF (p = 0.044), MMP-12 (p = 0.007), IL-6 (p = 0.0001), IL-8 (p = 0.0001) and MCP-1 (p = 0.0001) levels was observed in the AAD group compared to the control group. Furthermore, all AAD samples were positive for VEGF in the tunica media and showed vessel growth and immune-inflammatory infiltrate. A large number of cases (62.79%) showed inflammation at the edge of the dissection and approximately half (51.42%) showed neovessels growing at the edge of the dissection. Conclusions:The results suggest that VEGF-mediated angiogenesis and matrix degradation play a role in AAD. Finally, we believe that MMP-12 should be considered a marker of AAD. (C) 2013 S. Karger AG, Basel
2014
Atherosclerosis; Macrophages; Metalloprotease-12; Stanford type A acute aortic dissection; Acute Disease; Aneurysm, Dissecting; Antigens, CD31; Aortic Aneurysm, Thoracic; Cytokines; Female; Humans; Immunohistochemistry; Macrophages; Male; Matrix Metalloproteinase 12; Middle Aged; Tunica Media; Vascular Endothelial Growth Factor A; Cardiology and Cardiovascular Medicine; Pharmacology (medical)
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The multitasking role of macrophages in stanford type a acute aortic dissection / DEL PORTO, Flavia; DI GIOIA, Cira Rosaria Tiziana; Tritapepe, Luigi; Ferri, Livia; Leopizzi, Martina; Nofroni, Italo; Vincenzo De, Santis; DELLA ROCCA, Carlo; Mitterhofer, Anna Paola; Bruno, Guglielmo; Taurino, Maurizio; Maria, Proietta. - In: CARDIOLOGY. - ISSN 0008-6312. - 127:2(2014), pp. 123-129. [10.1159/000355253]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/557994
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