A new formulation (NF) of subcutaneous (sc) interferon (IFN) β-1a was developed in an attempt to improve injection tolerability and immunogenicity. We compared antiviral and IFNβ-stimulated gene (ISG) activities of IFNβ-1a sc NF with IFNβ-1a sc original formulation and IFNβ-1b sc. When equivalent unit amounts were compared, the IFNβ formulations demonstrated similar antiviral activity and induced similar levels of ISG mRNA. However, on a weight basis (ng/mL), significantly more IFNβ-1b sc was needed to equal the antiviral activity of either IFNβ-1a sc formulation, and both IFNβ-1a sc formulations induced significantly higher levels of ISG mRNA than IFNβ-1b sc. © 2014 Carolina Scagnolari, Carla Selvaggi, Emilia Di Biase, Maurizio Fraulo, Fernando Dangond, and Guido Antonelli.

In vitro assessment of the biologic activity of interferon beta formulations used for the treatment of relapsing multiple sclerosis / Scagnolari, Carolina; Selvaggi, Carla; Emilia Di, Biase; Maurizio, Fraulo; Fernando, Dangond; Antonelli, Guido. - In: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY. - ISSN 1532-1819. - STAMPA. - 35:3(2014), pp. 288-299. [10.1080/15321819.2013.848815]

In vitro assessment of the biologic activity of interferon beta formulations used for the treatment of relapsing multiple sclerosis

SCAGNOLARI, CAROLINA;SELVAGGI, CARLA;ANTONELLI, Guido
2014

Abstract

A new formulation (NF) of subcutaneous (sc) interferon (IFN) β-1a was developed in an attempt to improve injection tolerability and immunogenicity. We compared antiviral and IFNβ-stimulated gene (ISG) activities of IFNβ-1a sc NF with IFNβ-1a sc original formulation and IFNβ-1b sc. When equivalent unit amounts were compared, the IFNβ formulations demonstrated similar antiviral activity and induced similar levels of ISG mRNA. However, on a weight basis (ng/mL), significantly more IFNβ-1b sc was needed to equal the antiviral activity of either IFNβ-1a sc formulation, and both IFNβ-1a sc formulations induced significantly higher levels of ISG mRNA than IFNβ-1b sc. © 2014 Carolina Scagnolari, Carla Selvaggi, Emilia Di Biase, Maurizio Fraulo, Fernando Dangond, and Guido Antonelli.
2014
gene expression activity; in vitro assays; interferon beta-1a; sm; antiviral activity; nab; interferon beta-1b; biologic activity; ifn
01 Pubblicazione su rivista::01a Articolo in rivista
In vitro assessment of the biologic activity of interferon beta formulations used for the treatment of relapsing multiple sclerosis / Scagnolari, Carolina; Selvaggi, Carla; Emilia Di, Biase; Maurizio, Fraulo; Fernando, Dangond; Antonelli, Guido. - In: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY. - ISSN 1532-1819. - STAMPA. - 35:3(2014), pp. 288-299. [10.1080/15321819.2013.848815]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/557100
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