The developmental protein Numb is a major determinant of binary cell fates(1-3). It is also required for the differentiation of cerebellar granule cell progenitors (GCPs)(4) at a stage of development responsive to the morphogenic glycoprotein Hedehog(5,6). Hedgehog signalling is crucial for the physiological maintenance and self-renewal of neural stem cells and its deregulation is responsible for their progression towards tumorigenesis(5,7-11). The mechanisms that inhibit this pathway during the differentiation stage are poorly understood. Here, we identify Numb as a Hedgehog-pathway inhibitor that is downregulated in early GCPs and GCP-derived cancer cells. We demonstrate that the Hedgehog transcription factor Gli1 is targeted by Numb for Itch-dependent ubiquitination, which suppresses Hedgehog signals, thus arresting growth and promoting cell differentiation. This novel Numb-dependent regulatory loop may limit the extent and duration of Hedgehog signalling during neural-progenitor differentiation, and its subversion may be a relevant event in brain tumorigenesis.
Numb is a suppressor of Hedgehog signaling and targets Gli1 for Itch-dependent ubiquitination / DI MARCOTULLIO, L., Ferretti, E., Greco, A., DE SMAELE, E., Po, A., Alimandi, M., Giannini, G., Maroder, M., Screpanti, I., AND GULINO, A.. - ELETTRONICO. - (2006). (EMBO Workshop Hedgehog-Gli signaling in cancer and stem cells Roma ).
Numb is a suppressor of Hedgehog signaling and targets Gli1 for Itch-dependent ubiquitination.
FERRETTI, ELISABETTA;DE SMAELE, Enrico;PO, AGNESE;ALIMANDI, MAURIZIO;GIANNINI G;SCREPANTI I;
2006
Abstract
The developmental protein Numb is a major determinant of binary cell fates(1-3). It is also required for the differentiation of cerebellar granule cell progenitors (GCPs)(4) at a stage of development responsive to the morphogenic glycoprotein Hedehog(5,6). Hedgehog signalling is crucial for the physiological maintenance and self-renewal of neural stem cells and its deregulation is responsible for their progression towards tumorigenesis(5,7-11). The mechanisms that inhibit this pathway during the differentiation stage are poorly understood. Here, we identify Numb as a Hedgehog-pathway inhibitor that is downregulated in early GCPs and GCP-derived cancer cells. We demonstrate that the Hedgehog transcription factor Gli1 is targeted by Numb for Itch-dependent ubiquitination, which suppresses Hedgehog signals, thus arresting growth and promoting cell differentiation. This novel Numb-dependent regulatory loop may limit the extent and duration of Hedgehog signalling during neural-progenitor differentiation, and its subversion may be a relevant event in brain tumorigenesis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
