Adipose-Derived Stem Cell: an Innovative Therapeutic Approach in Systemic Sclerosis and Parry-Romberg Syndrome

INTRODUCTION: Cell-based therapies represent a promising therapeutic approach to enhance the regeneration of damaged tissue and the combination with specific soluble mediators and biomaterial scaffolds has allowed the introduction of new treatment strategies in regenerative medicine. Adipose-derived stem cells (ASCs) show the ability to differentiate into several cell lineages such as chondrocytes, osteoblasts, adipocytes, neuronal cells, and muscle cells. These cells are abundant in normal human fat and they can be easily harvested from small amount of liposuction. For these reasons, they are greatly employed in the treatment of cutaneous and musculoskeletal defects are greatly. MATERIALS AND METHODS: ASCs isolated from liposuction aspirate by type I collagenases digestion were successively characterized by FACS analysis in order to verify their mesenchymal origin and test their capacity to differentiate into adipogenic, chondrogenic and osteogenic lineages. Autologous cultu