The interaction of doxorubicin (DX) with model polynucleotides poly(dG-dC)center dot poly(dG-dC) (polyGC), poly(dA-dT)center dot poly(dA-dT) (polyAT), and calf thymus DNA has been studied by several spectroscopic techniques in phosphate buffer aqueous solutions. UV-vis, circular dichroism, and fluorescence spectroscopic data confirm that intercalation is the prevailing mode of interaction, and also reveal that the interaction with AT-rich regions leads to the transfer of excitation energy to DX not previously documented in the literature. Moreover, the DX affinity for AT sites has been found to be on the same order of magnitude as that reported for GC sites.
Interaction of Doxorubicin with Polynucleotides. A Spectroscopic Study / Marta, Airoldi; Giampaolo, Barone; Giuseppe, Gennaro; Anna Maria, Giuliani; Giustini, Mauro. - In: BIOCHEMISTRY. - ISSN 0006-2960. - STAMPA. - 53:13(2014), pp. 2197-2207. [10.1021/bi401687v]
Interaction of Doxorubicin with Polynucleotides. A Spectroscopic Study
GIUSTINI, Mauro
2014
Abstract
The interaction of doxorubicin (DX) with model polynucleotides poly(dG-dC)center dot poly(dG-dC) (polyGC), poly(dA-dT)center dot poly(dA-dT) (polyAT), and calf thymus DNA has been studied by several spectroscopic techniques in phosphate buffer aqueous solutions. UV-vis, circular dichroism, and fluorescence spectroscopic data confirm that intercalation is the prevailing mode of interaction, and also reveal that the interaction with AT-rich regions leads to the transfer of excitation energy to DX not previously documented in the literature. Moreover, the DX affinity for AT sites has been found to be on the same order of magnitude as that reported for GC sites.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.