An NMR analytical protocol based on complementary high and low field measurements is proposed for blueberry characterization. Untargeted NMR metabolite profiling of blueberries aqueous and organic extracts as well as targeted NMR analysis focused on anthocyanins and other phenols are reported. Bligh-Dyer and microwave-assisted extractions were carried out and compared showing a better recovery of lipidic fraction in the case of microwave procedure. Water-soluble metabolites belonging to different classes such as sugars, amino acids, organic acids, and phenolic compounds, as well as metabolites soluble in organic solvent such as triglycerides, sterols, and fatty acids, were identified. Five anthocyanins (malvidin-3-glucoside, malvidin-3-galactoside, delphinidin-3-glucoside, delphinidin-3-galactoside, and petunidin-3-glucoside) and 3-O- -L-rhamnopyranosyl quercetin were identified in solid phase extract. The water status of fresh and withered blueberries was monitored by portable NMR and fast-field cycling NMR. 1Hdepth profiles, T2 transverse relaxation times and dispersion profiles were found to be sensitive to the withering
NMR methodologies in the analysis of blueberries / Donatella, Capitani; Anatoly P., Sobolev; Delfini, Maurizio; Vista, Silvia; Antiochia, Riccarda; Noemi, Proietti; Salvatore, Bubici; Gianni, Ferrante; Carradori, Simone; Flavio Roberto De, Salvador; Mannina, Luisa. - In: ELECTROPHORESIS. - ISSN 0173-0835. - ELETTRONICO. - 35:11(2014), pp. 1615-1626. [10.1002/elps.201300629]
NMR methodologies in the analysis of blueberries
DELFINI, Maurizio;VISTA, SILVIA;ANTIOCHIA, RICCARDA;CARRADORI, Simone;MANNINA, LUISA
2014
Abstract
An NMR analytical protocol based on complementary high and low field measurements is proposed for blueberry characterization. Untargeted NMR metabolite profiling of blueberries aqueous and organic extracts as well as targeted NMR analysis focused on anthocyanins and other phenols are reported. Bligh-Dyer and microwave-assisted extractions were carried out and compared showing a better recovery of lipidic fraction in the case of microwave procedure. Water-soluble metabolites belonging to different classes such as sugars, amino acids, organic acids, and phenolic compounds, as well as metabolites soluble in organic solvent such as triglycerides, sterols, and fatty acids, were identified. Five anthocyanins (malvidin-3-glucoside, malvidin-3-galactoside, delphinidin-3-glucoside, delphinidin-3-galactoside, and petunidin-3-glucoside) and 3-O- -L-rhamnopyranosyl quercetin were identified in solid phase extract. The water status of fresh and withered blueberries was monitored by portable NMR and fast-field cycling NMR. 1Hdepth profiles, T2 transverse relaxation times and dispersion profiles were found to be sensitive to the witheringI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
