Purpose: A prototype of an atmospheric pressure cold plasma (APCP) source has been developed for the treatment of corneal infections and for ocular surface sterilization. Two minutes of APCP treatment was previously found effective against various microorganisms but caused, in corneal fibroblast cultures, transitory ROS formation, expression of the ogg1 marker of oxidative damage and increase of apoptosis. In the present study we investigated the transcriptome changes of whole human cornea (HC) ex vivo exposed to the disinfectant dose of APCP. Methods: The effects of exposure to APCP for 2 min on HC, in the absence or presence of the antioxidant N-acetyl L-cysteine (NAC) and related to unexposed controls were assessed at 6 h post-treatment by histological and immunohistochemical analysis, Western blotting and Illlumina RNA sequencing (RNA-seq). Additional HC were subsequently exposed to APCP in the same conditions to measure the expression o
Antimicrobial Peptides and Acanthamoeba: Peptide expression and anti-amoebicidal activity via time-lapse imaging / Joseph C., Manarang; Hasna, Baidour; Satya Sree N., Kolar; Mangoni, Maria Luisa; Alison M., Mcdermott. - ELETTRONICO. - (2014). (Intervento presentato al convegno Annual Meeting of the Association of Research in Vision and Ophthalmology tenutosi a Orlando nel May 4-8, 2014).
Antimicrobial Peptides and Acanthamoeba: Peptide expression and anti-amoebicidal activity via time-lapse imaging
MANGONI, Maria Luisa;
2014
Abstract
Purpose: A prototype of an atmospheric pressure cold plasma (APCP) source has been developed for the treatment of corneal infections and for ocular surface sterilization. Two minutes of APCP treatment was previously found effective against various microorganisms but caused, in corneal fibroblast cultures, transitory ROS formation, expression of the ogg1 marker of oxidative damage and increase of apoptosis. In the present study we investigated the transcriptome changes of whole human cornea (HC) ex vivo exposed to the disinfectant dose of APCP. Methods: The effects of exposure to APCP for 2 min on HC, in the absence or presence of the antioxidant N-acetyl L-cysteine (NAC) and related to unexposed controls were assessed at 6 h post-treatment by histological and immunohistochemical analysis, Western blotting and Illlumina RNA sequencing (RNA-seq). Additional HC were subsequently exposed to APCP in the same conditions to measure the expression oI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
