Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of kinetic quiescent cells, frequently resistant to tyrosine kinase inhibitors (TKIs). The Bcr-Abl oncogene and the resulting fusion protein, in fact, activates multiple cross-talking signal transduction pathways (STP), such as RAS/MEK/ERK, PI3K/Akt, Wnt and STAT5, potentially contributing to TKIs drug resistance. Since increasing evidence reports the cooperation of numerous STP in the control of cell proliferation and survival in CML, the aim of this project was to analyze, at the protein level, the expression and activation profile of various STP involved in the mechanisms of cell proliferation and survival of CML CD34+ cells, as compared to different sources of normal CD34+ cells. CD34+ cells were purified by immunomagnetic separation from peripheral blood (PB) of 7 newly diagnosed chronic phase (CP) CML patients and compared to the normal counterpart obtained from normal bone marrow of three health
Proteomic profile of CD34+ cells from chronic myeloid leukemia patients and from normal donors / Ricciardi, Maria Rosaria; V., Salvestrini; M., Forcato; Licchetta, Roberto; Mirabilii, Simone; L., Rossi; Allegretti, Matteo; M., Vincenzi; S., Salati; F., Castagnetti; G., Rosti; G., Alimena; R., Manfredini; S., Bicciato; R. M., Lemoli; Tafuri, Agostino. - In: BLOOD. - ISSN 0006-4971. - ELETTRONICO. - 122:(2013). (Intervento presentato al convegno American Society of Hematology (ASH) 55th Annual Meeting tenutosi a New Orleans, Louisiana, USA nel December 7 - 10, 2013).
Proteomic profile of CD34+ cells from chronic myeloid leukemia patients and from normal donors
RICCIARDI, Maria Rosaria;LICCHETTA, ROBERTO;MIRABILII, SIMONE;ALLEGRETTI, MATTEO;TAFURI, Agostino
2013
Abstract
Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of kinetic quiescent cells, frequently resistant to tyrosine kinase inhibitors (TKIs). The Bcr-Abl oncogene and the resulting fusion protein, in fact, activates multiple cross-talking signal transduction pathways (STP), such as RAS/MEK/ERK, PI3K/Akt, Wnt and STAT5, potentially contributing to TKIs drug resistance. Since increasing evidence reports the cooperation of numerous STP in the control of cell proliferation and survival in CML, the aim of this project was to analyze, at the protein level, the expression and activation profile of various STP involved in the mechanisms of cell proliferation and survival of CML CD34+ cells, as compared to different sources of normal CD34+ cells. CD34+ cells were purified by immunomagnetic separation from peripheral blood (PB) of 7 newly diagnosed chronic phase (CP) CML patients and compared to the normal counterpart obtained from normal bone marrow of three healthI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
