In this paper, an electromagnetic characterization of amoveable wire electrode microchamber for nanosecond pulse delivery is proposed. The characterization of the exposure system was carried out through experimental measurements and numerical simulations. The frequency and time domain analyses demonstrate the utility of the proposed assembly for delivering pulses as short as 2.5 ns. High-voltage measurements (similar to 1.2 kV) were also performed using pulse generators based on two different technologies with applied pulse durations of 5.0 and 2.5 ns. Validation of the delivery system was accomplished with biological experiments involving cell electroporation with 2.5 and 5.0 ns, 10-MV/m pulsed electric fields. A dose-dependent area increase (osmotic swelling) of the Jurkat cells was observed with pulses as short as 2.5 ns.
Moveable Wire Electrode Microchamber for Nanosecond Pulsed Electric-Field Delivery / Yu Hsuan, Wu; Delia Arnaud, Cormos; Casciola, Maura; Jason M., Sanders; Leveque, Philippe; P., Thomas Vernier. - In: IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING. - ISSN 0018-9294. - 60:2(2013), pp. 489-496. [10.1109/tbme.2012.2228650]
Moveable Wire Electrode Microchamber for Nanosecond Pulsed Electric-Field Delivery
CASCIOLA, MAURA;
2013
Abstract
In this paper, an electromagnetic characterization of amoveable wire electrode microchamber for nanosecond pulse delivery is proposed. The characterization of the exposure system was carried out through experimental measurements and numerical simulations. The frequency and time domain analyses demonstrate the utility of the proposed assembly for delivering pulses as short as 2.5 ns. High-voltage measurements (similar to 1.2 kV) were also performed using pulse generators based on two different technologies with applied pulse durations of 5.0 and 2.5 ns. Validation of the delivery system was accomplished with biological experiments involving cell electroporation with 2.5 and 5.0 ns, 10-MV/m pulsed electric fields. A dose-dependent area increase (osmotic swelling) of the Jurkat cells was observed with pulses as short as 2.5 ns.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
