The artificial enhancing of the uptake, transport or delivery of oxygen, including the intake of modified hemoglobin products (e.g. HBOCs) is a prohibited method included in the WADA List of Prohibited Substances (M1. Manipulation of Blood and Blood Components). In this work we present a fast screening to detect liposome encapsulated hemoglobins (LEH). LEH can be detected in serum/plasma using flow cytometry-based techniques with a dual color staining procedure that requires several hours for sample preparation and analysis. The aim of this work is to test the potential of a cytometry-based hematological analyzer such as ADVIA 120 (Siemens) for a rapid screening of the presence of liposomes within a serum/plasma sample.
Fast screening for liposome encapsulated hemoglobin (LEH) using ADVIA 120 flow cytometry-based hematological analyzer / Donati, Francesco; Colicchia, Sonia; de la Torre, X.; Iannella, L.; Botre', Francesco. - STAMPA. - 21:(2013), pp. 161-163. (Intervento presentato al convegno Manfred Donike Workshop tenutosi a Cologne nel FEBBRAIO-MARZO 2013).
Fast screening for liposome encapsulated hemoglobin (LEH) using ADVIA 120 flow cytometry-based hematological analyzer
DONATI, Francesco;COLICCHIA, SONIA;L. Iannella;BOTRE', Francesco
2013
Abstract
The artificial enhancing of the uptake, transport or delivery of oxygen, including the intake of modified hemoglobin products (e.g. HBOCs) is a prohibited method included in the WADA List of Prohibited Substances (M1. Manipulation of Blood and Blood Components). In this work we present a fast screening to detect liposome encapsulated hemoglobins (LEH). LEH can be detected in serum/plasma using flow cytometry-based techniques with a dual color staining procedure that requires several hours for sample preparation and analysis. The aim of this work is to test the potential of a cytometry-based hematological analyzer such as ADVIA 120 (Siemens) for a rapid screening of the presence of liposomes within a serum/plasma sample.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.