When nanoparticles (NPs) are exposed to biological fluids they adsorb biomolecules (mainly proteins) to form a protein corona. We have applied nanoliquid-cromatography mass spectroscopy (NanoLC-MS/MS) to quantitatively determine the proteins associated with several lipid NP formulations after incubation with human plasma (HP). By NanoLC-MS/MS we identified vitronectin as the most promising protein corona component for active targeting. To exploit the "protein corona effect" we investigated the interactions of l, 2-dioleoyl-3-trimethylammonium propane (DOTAP)/DNA cationic liposome/DNA complexes (lipoplexes) with target cells. By applying two-color fluorescence laser scanning confocal microscopy (LSCM) we demonstrate that vitronectin directs a receptor-mediated uptake of lipoplexes into target cells. This experiment sets the basis for a rational exploitation of the protein corona for targeted drug and gene delivery.

Exploiting the "protein corona effect" for targeted drug and gene delivery / CARACCIOLO, Giulio; POZZI, DANIELA; CAPRIOTTI, ANNA LAURA; CAVALIERE, CHIARA; F., Cardarelli; F., Salomone; LAGANA', Aldo. - STAMPA. - 3(2013), pp. 247-250. ((Intervento presentato al convegno Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 tenutosi a Washington; United States nel 12 May 2013 through 16 May 2013.

Exploiting the "protein corona effect" for targeted drug and gene delivery

CARACCIOLO, Giulio;POZZI, DANIELA;CAPRIOTTI, ANNA LAURA;CAVALIERE, CHIARA;LAGANA', Aldo
2013

Abstract

When nanoparticles (NPs) are exposed to biological fluids they adsorb biomolecules (mainly proteins) to form a protein corona. We have applied nanoliquid-cromatography mass spectroscopy (NanoLC-MS/MS) to quantitatively determine the proteins associated with several lipid NP formulations after incubation with human plasma (HP). By NanoLC-MS/MS we identified vitronectin as the most promising protein corona component for active targeting. To exploit the "protein corona effect" we investigated the interactions of l, 2-dioleoyl-3-trimethylammonium propane (DOTAP)/DNA cationic liposome/DNA complexes (lipoplexes) with target cells. By applying two-color fluorescence laser scanning confocal microscopy (LSCM) we demonstrate that vitronectin directs a receptor-mediated uptake of lipoplexes into target cells. This experiment sets the basis for a rational exploitation of the protein corona for targeted drug and gene delivery.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/527793
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