The nutritional and cancer-protective properties of the oil extracted mechanically from the ripe fruits of Olea europaea trees are attracting constantly more attention worldwide. The preparation of high-quality protein samples from plant tissues for proteomic analysis poses many challenging problems. In this study we employed a proteomic platform based on two different extraction methods, SDS and CHAPS based protocols, followed by two precipitation protocols, TCA/acetone and MeOH precipitation, in order to increase the final number of identified proteins. The use of advanced MS techniques in combination with the Swissprot and NCBI Viridiplantae databases and TAIR10 Arabidopsis database allowed us to identify 1265 proteins, of which 22 belong to O. europaea. The application of this proteomic platform for protein extraction and identification will be useful also for other proteomic studies on recalcitrant plant/fruit tissues. (C) 2013 Published by Elsevier B.V.

Proteomic platform for the identification of proteins in olive (Olea europaea) pulp / Capriotti, ANNA LAURA; Cavaliere, Chiara; Foglia, Patrizia; Piovesana, Susy; Samperi, Roberto; Stampachiacchiere, Serena; Lagana', Aldo. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 800:(2013), pp. 36-42. [10.1016/j.aca.2013.09.014]

Proteomic platform for the identification of proteins in olive (Olea europaea) pulp

CAPRIOTTI, ANNA LAURA;CAVALIERE, CHIARA;FOGLIA, Patrizia;PIOVESANA, SUSY;SAMPERI, Roberto;STAMPACHIACCHIERE, SERENA;LAGANA', Aldo
2013

Abstract

The nutritional and cancer-protective properties of the oil extracted mechanically from the ripe fruits of Olea europaea trees are attracting constantly more attention worldwide. The preparation of high-quality protein samples from plant tissues for proteomic analysis poses many challenging problems. In this study we employed a proteomic platform based on two different extraction methods, SDS and CHAPS based protocols, followed by two precipitation protocols, TCA/acetone and MeOH precipitation, in order to increase the final number of identified proteins. The use of advanced MS techniques in combination with the Swissprot and NCBI Viridiplantae databases and TAIR10 Arabidopsis database allowed us to identify 1265 proteins, of which 22 belong to O. europaea. The application of this proteomic platform for protein extraction and identification will be useful also for other proteomic studies on recalcitrant plant/fruit tissues. (C) 2013 Published by Elsevier B.V.
2013
high resolution mass spectrometry; olive pulp; gel-free approach; protein extraction; plant proteomics
01 Pubblicazione su rivista::01a Articolo in rivista
Proteomic platform for the identification of proteins in olive (Olea europaea) pulp / Capriotti, ANNA LAURA; Cavaliere, Chiara; Foglia, Patrizia; Piovesana, Susy; Samperi, Roberto; Stampachiacchiere, Serena; Lagana', Aldo. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 800:(2013), pp. 36-42. [10.1016/j.aca.2013.09.014]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/525851
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