The tumor suppressor p53 plays a central role in the regulation of cellular growth and apoptosis. In the yeast Saccharomyces cerevisiae, the overexpression of the human p53 leads to growth inhibition and apoptotic cell death on minimal medium. In the present work, we show that p53-expressing cells are more susceptible to cell death after an apoptotic stimulus such as H2O2. The analysis of mutants involved in yeast apoptosis-like death suggests that the observed cell death is Yca1 independent and mainly mediated through Nuc1p. © 2013 Federation of European Microbiological Societies.
p53 death signal is mainly mediated by Nuc1(EndoG) in the yeast Saccharomyces cerevisiae / Palermo, Vanessa; Mangiapelo, Eleonora; Piloto, Cristina; Luisa, Pieri; Muscolini, Michela; Tuosto, Loretta; Mazzoni, Cristina. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - ELETTRONICO. - 13:7(2013), pp. 682-688. [10.1111/1567-1364.12067]
p53 death signal is mainly mediated by Nuc1(EndoG) in the yeast Saccharomyces cerevisiae
PALERMO, Vanessa;MANGIAPELO, ELEONORA;PILOTO, CRISTINA;MUSCOLINI, MICHELA;TUOSTO, Loretta;MAZZONI, Cristina
2013
Abstract
The tumor suppressor p53 plays a central role in the regulation of cellular growth and apoptosis. In the yeast Saccharomyces cerevisiae, the overexpression of the human p53 leads to growth inhibition and apoptotic cell death on minimal medium. In the present work, we show that p53-expressing cells are more susceptible to cell death after an apoptotic stimulus such as H2O2. The analysis of mutants involved in yeast apoptosis-like death suggests that the observed cell death is Yca1 independent and mainly mediated through Nuc1p. © 2013 Federation of European Microbiological Societies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
