This protocol describes paraformaldehyde fixation of Drosophila testes. The procedure preserves F-actincontaining structures because it does not involve the use of methanol. Thus, it is particularly suitable for contractile ring and fusome visualization. However, it does not preserve cell morphology and microtubules as efficiently as methanol-acetone fixation and does not permit clear detection of the Y loops. © 2012 Cold Spring Harbor Laboratory Press.
Paraformaldehyde fixation of drosophila testes / Bonaccorsi, Silvia; M. g., Giansanti; Cenci, Giovanni; Gatti, Maurizio. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2012:1(2012), pp. pdb.prot067330-pdb.prot067330. [10.1101/pdb.prot067330]
Paraformaldehyde fixation of drosophila testes
BONACCORSI, Silvia;CENCI, GIOVANNI;GATTI, MAURIZIO
2012
Abstract
This protocol describes paraformaldehyde fixation of Drosophila testes. The procedure preserves F-actincontaining structures because it does not involve the use of methanol. Thus, it is particularly suitable for contractile ring and fusome visualization. However, it does not preserve cell morphology and microtubules as efficiently as methanol-acetone fixation and does not permit clear detection of the Y loops. © 2012 Cold Spring Harbor Laboratory Press.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
