This protocol describes chromatin staining of Drosophila testes. To visualize DNA, preparations fixed using methanol-acetone, paraformaldehyde, or formaldehyde can be stained with several DNAbinding dyes. If the slides are to be examined with a fluorescence microscope equipped with filters that permit ultraviolet (UV) excitation, suitable dyes for DNA staining are Hoechst 33258 or 4′,6- diamidino-2-phenylindole (DAPI). If the slides are to be analyzed with a confocal microscope not equipped with a UV laser, DNA can be stained with either propidium iodide or TOTO-3 iodide. © 2012 Cold Spring Harbor Laboratory Press.
Chromatin staining of Drosophila testes / Bonaccorsi, Silvia; M. g., Giansanti; Cenci, Giovanni; Gatti, Maurizio. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2012:8(2012), pp. pdb.prot067363-pdb.prot067363. [10.1101/pdb.prot067363]
Chromatin staining of Drosophila testes
BONACCORSI, Silvia;CENCI, GIOVANNI;GATTI, MAURIZIO
2012
Abstract
This protocol describes chromatin staining of Drosophila testes. To visualize DNA, preparations fixed using methanol-acetone, paraformaldehyde, or formaldehyde can be stained with several DNAbinding dyes. If the slides are to be examined with a fluorescence microscope equipped with filters that permit ultraviolet (UV) excitation, suitable dyes for DNA staining are Hoechst 33258 or 4′,6- diamidino-2-phenylindole (DAPI). If the slides are to be analyzed with a confocal microscope not equipped with a UV laser, DNA can be stained with either propidium iodide or TOTO-3 iodide. © 2012 Cold Spring Harbor Laboratory Press.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
