Telomeres are specialized structures at the end of eukaryotic chromosomes that are required to preserve genome integrity, chromosome stability and nuclear architecture. Telomere maintenance and function are established epigenetically in several eukaryotes. However, the exact chromatin enzymatic modifications regulating telomere homeostasis are poorly understood. In Drosophila melanogaster, telomere length and stability are maintained through the retrotransposition of specialized telomeric sequences and by the specific loading of protecting capping proteins, respectively. Here, we show that the loss of the essential and evolutionarily conserved histone deacetylase Rpd3, the homolog of mammalian HDAC1, causes aberrant telomeric fusions on polytene chromosome ends. Remarkably, these telomere fusion defects are associated with a marked decrease of histone H4 acetylation, as well as an accumulation of heterochromatic epigenetic marks at telomeres, including histone H3K9 trimethylation and the heterochromatic protein HP2. Our work suggests that Drosophila telomere structure is epigenetically regulated by the histone deacetylase Rpd3. © 2011. Published by The Company of Biologists Ltd.

The histone deacetylase Rpd3 regulates the heterochromatin structure of Drosophila telomeres / G. b., Burgio; Cipressa, Francesca; A. b., Ingrassia; Cenci, Giovanni; D. b., Corona. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - STAMPA. - 124:12(2011), pp. 2041-2048. [10.1242/jcs.078261]

The histone deacetylase Rpd3 regulates the heterochromatin structure of Drosophila telomeres

CIPRESSA, FRANCESCA;CENCI, GIOVANNI;
2011

Abstract

Telomeres are specialized structures at the end of eukaryotic chromosomes that are required to preserve genome integrity, chromosome stability and nuclear architecture. Telomere maintenance and function are established epigenetically in several eukaryotes. However, the exact chromatin enzymatic modifications regulating telomere homeostasis are poorly understood. In Drosophila melanogaster, telomere length and stability are maintained through the retrotransposition of specialized telomeric sequences and by the specific loading of protecting capping proteins, respectively. Here, we show that the loss of the essential and evolutionarily conserved histone deacetylase Rpd3, the homolog of mammalian HDAC1, causes aberrant telomeric fusions on polytene chromosome ends. Remarkably, these telomere fusion defects are associated with a marked decrease of histone H4 acetylation, as well as an accumulation of heterochromatic epigenetic marks at telomeres, including histone H3K9 trimethylation and the heterochromatic protein HP2. Our work suggests that Drosophila telomere structure is epigenetically regulated by the histone deacetylase Rpd3. © 2011. Published by The Company of Biologists Ltd.
2011
animals; article; chromosome aberration; chromosome structure; drosophila; drosophila melanogaster; drosophila proteins; enzyme activity; epigenetics; epigenomics; eukaryota; heterochromatin; histone acetylation; histone deacetylase 1; histone deacetylase rpd 3; histone methylation; male; mammalia; nonhuman; polytene chromosome; priority journal; regulatory mechanism; telomere; unclassified drug
01 Pubblicazione su rivista::01a Articolo in rivista
The histone deacetylase Rpd3 regulates the heterochromatin structure of Drosophila telomeres / G. b., Burgio; Cipressa, Francesca; A. b., Ingrassia; Cenci, Giovanni; D. b., Corona. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - STAMPA. - 124:12(2011), pp. 2041-2048. [10.1242/jcs.078261]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/519659
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