Good mitotic chromosome preparations are essential for the immunolocalization of chromosomal proteins. Although methanol/acetic acid fixation techniques preserve chromosome morphology very well, they remove a substantial fraction of chromosomal proteins. We have developed fixation/immunostaining procedures, described here, that are suitable for the immunolocalization of proteinaceous components of metaphase chromosomes from larval Drosophila brain cells. These procedures result in good chromosomal quality with minimal removal of proteins.
Immunostaining of mitotic chromosomes from Drosophila larval brain / Pimpinelli, Sergio; Bonaccorsi, Silvia; Fanti, Laura; Gatti, Maurizio. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - ELETTRONICO. - 2011:9(2011), pp. 1105-1109. [10.1101/pdb.prot065524]
Immunostaining of mitotic chromosomes from Drosophila larval brain
PIMPINELLI, Sergio;BONACCORSI, Silvia;FANTI, Laura;GATTI, MAURIZIO
2011
Abstract
Good mitotic chromosome preparations are essential for the immunolocalization of chromosomal proteins. Although methanol/acetic acid fixation techniques preserve chromosome morphology very well, they remove a substantial fraction of chromosomal proteins. We have developed fixation/immunostaining procedures, described here, that are suitable for the immunolocalization of proteinaceous components of metaphase chromosomes from larval Drosophila brain cells. These procedures result in good chromosomal quality with minimal removal of proteins.| File | Dimensione | Formato | |
|---|---|---|---|
|
Pimpinelli_Immunostaining_2011.pdf
solo gestori archivio
Tipologia:
Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
235.1 kB
Formato
Adobe PDF
|
235.1 kB | Adobe PDF | Contatta l'autore |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
