This study examined for the first time the effect of low-energy ultrasound (US), used alone or in combination with methyl jasmonate (MeJA), on viniferin production in cell cultures of Vitis vinifera L. cv Alphonse Lavallee. Cell suspensions were exposed for 2 min to US (power 30, 60, and 90 mW cm(-3)). The highest viniferin production was obtained at 30 mW cm(-3). When sonication was performed twice, the effect on viniferin production was negligible, whereas triple sonication slightly increased production. US treatment at 30 mW cm(-3) for 5 min decreased viniferin production and induced cellular death. The combined use of MeJA and US (2 min) increased the production of delta-viniferin, the dominant stilbene, more than each elicitor used alone. These results suggest that low-energy US, alone and in combination with MeJA, can act as a physical elicitor to stimulate viniferin production in V. vinifera cell cultures.
Enhancement of Viniferin Production in Vitis vinifera L. cv. Alphonse Lavallee Cell Suspensions by Low-Energy Ultrasound Alone and in Combination with Methyl Jasmonate / Santamaria, Anna Rita; Marzia, Innocenti; Nadia, Mulinacci; Fabrizio, Melani; Valletta, Alessio; Ilaria, Sciandra; Pasqua, Gabriella. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - STAMPA. - 60:44(2012), pp. 11135-11142. [10.1021/jf301936u]