Objectives. Montgomery Safe-T-Tube deterioration and early biofilm colonization may explain the discomfort claimed by many patients and clinical failures. The aim of the study was to analyze the deterioration of Montgomery Safe-T-Tube morphological and mechanical properties in vivo in 16 patients by using microbiological methods, optical and electron microscopy, and engineering tests. Study Design. Prospective controlled study at a single medical center. Setting. University hospital. Subjects and Methods. The study, conducted from April 2007 to February 2012 at the "Sapienza" University of Rome, was designed to collect 2 Montgomery Safe-T-Tubes from each patient. The first was removed 3 to 15 days after insertion (group A) and the second at least 90 days after (group B). Specimens underwent microbiologic assays, electron microscopic analysis, immunocytologic analysis, and mechanical tests. Results. Microorganisms were not isolated in 2 group A cases (12%), whereas they were in all group B cases. Biofilm was identified in 11 of 16 (69%) group A samples and in 16 of 16 (100%) group B samples (P =.0149) using scanning electron microscopy. Immunohistochemistry showed monocytegranulocyte line cells producing interleukin-1b on the external surfaces of Montgomery Safe-T-Tubes. The tensile test showed that the wear related to the longer period of use makes Montgomery Safe-T-Tubes more rigid than newer ones. Conclusion. Early biofilm colonization takes place in Montgomery Safe-T-Tubes in most cases. The mechanical decay could be justified in part by the destructive biofilm activity and by the release of inflammatory effectors and enzymes.

Is Montgomery Tracheal Safe-T-Tube Clinical Failure Induced by Biofilm? / Fusconi, Massimo; LO VASCO, VINCENZA RITA; Delfini, A.; DE VIRGILIO, Armando; Taddei, A. R.; Vassalli, C.; Conte, Michela; Del Sette, F.; Benincasa, A. T.; DE VINCENTIIS, Marco. - In: OTOLARYNGOLOGY-HEAD AND NECK SURGERY. - ISSN 0194-5998. - STAMPA. - 149:2(2013), pp. 269-276. [10.1177/0194599813488752]

Is Montgomery Tracheal Safe-T-Tube Clinical Failure Induced by Biofilm?

FUSCONI, Massimo;LO VASCO, VINCENZA RITA;A. Delfini;DE VIRGILIO, ARMANDO;CONTE, MICHELA;A. T. Benincasa;DE VINCENTIIS, Marco
2013

Abstract

Objectives. Montgomery Safe-T-Tube deterioration and early biofilm colonization may explain the discomfort claimed by many patients and clinical failures. The aim of the study was to analyze the deterioration of Montgomery Safe-T-Tube morphological and mechanical properties in vivo in 16 patients by using microbiological methods, optical and electron microscopy, and engineering tests. Study Design. Prospective controlled study at a single medical center. Setting. University hospital. Subjects and Methods. The study, conducted from April 2007 to February 2012 at the "Sapienza" University of Rome, was designed to collect 2 Montgomery Safe-T-Tubes from each patient. The first was removed 3 to 15 days after insertion (group A) and the second at least 90 days after (group B). Specimens underwent microbiologic assays, electron microscopic analysis, immunocytologic analysis, and mechanical tests. Results. Microorganisms were not isolated in 2 group A cases (12%), whereas they were in all group B cases. Biofilm was identified in 11 of 16 (69%) group A samples and in 16 of 16 (100%) group B samples (P =.0149) using scanning electron microscopy. Immunohistochemistry showed monocytegranulocyte line cells producing interleukin-1b on the external surfaces of Montgomery Safe-T-Tubes. The tensile test showed that the wear related to the longer period of use makes Montgomery Safe-T-Tubes more rigid than newer ones. Conclusion. Early biofilm colonization takes place in Montgomery Safe-T-Tubes in most cases. The mechanical decay could be justified in part by the destructive biofilm activity and by the release of inflammatory effectors and enzymes.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/516528
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