The Na+ ionophore monensin affects cellular pH and, depending on its concentration, causes the survival or death of tumor cells. In the present study, we elucidated the survival pathway activated in U937 cells, a human lymphoma-derived cell line. These cells treated with monensin at a concentration of 5 μM were growth-arrested in G1, activated p38 mitogen-activated protein kinase (MAPK) and showed an increased expression of cyclooxygenase-2 (COX-2). The latter two molecular events were linked, as pharmacological inhibition of the MAPK did not allow COX-2 increased expression. Furthermore, we showed that p38 and COX-2 keep monensin-stressed U937 cells alive, as pharmacological inhibition of each enzyme caused cell death.

Cyclooxygenase-2 is induced by p38 MAPK and promotes cell survival / R., Parente; E., Trifiro; F., Cuozzo; S., Valia; Cirone, Mara; DI RENZO, Livia Maria. - In: ONCOLOGY REPORTS. - ISSN 1021-335X. - ELETTRONICO. - 29:5(2013), pp. 1999-2004. [10.3892/or.2013.2308]

Cyclooxygenase-2 is induced by p38 MAPK and promotes cell survival

CIRONE, Mara;DI RENZO, Livia Maria
2013

Abstract

The Na+ ionophore monensin affects cellular pH and, depending on its concentration, causes the survival or death of tumor cells. In the present study, we elucidated the survival pathway activated in U937 cells, a human lymphoma-derived cell line. These cells treated with monensin at a concentration of 5 μM were growth-arrested in G1, activated p38 mitogen-activated protein kinase (MAPK) and showed an increased expression of cyclooxygenase-2 (COX-2). The latter two molecular events were linked, as pharmacological inhibition of the MAPK did not allow COX-2 increased expression. Furthermore, we showed that p38 and COX-2 keep monensin-stressed U937 cells alive, as pharmacological inhibition of each enzyme caused cell death.
2013
arachidonic acid; cox-2; cyclooxygenase-2; mapk; monensin; p38; p38 mapk; survival; survival pathway
01 Pubblicazione su rivista::01a Articolo in rivista
Cyclooxygenase-2 is induced by p38 MAPK and promotes cell survival / R., Parente; E., Trifiro; F., Cuozzo; S., Valia; Cirone, Mara; DI RENZO, Livia Maria. - In: ONCOLOGY REPORTS. - ISSN 1021-335X. - ELETTRONICO. - 29:5(2013), pp. 1999-2004. [10.3892/or.2013.2308]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/513986
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