Pseudomonas aeruginosa is an opportunistic bacterial pathogen that forms sessile communities, named biofilms. The non-motile forms are very difficult to eradicate and are often associated with the establishment of persistent infections, especially in patients with cystic fibrosis. The resistance of P. aeruginosa to conventional antibiotics has become a growing health concern worldwide and has prompted the search for new anti-infective agents with new modes of action. Naturally occurring antimicrobial peptides (AMPs) represent promising future template candidates. Here we report on the potent activity and membrane-perturbing effects of the amphibian AMP esculentin(1-21), on both the free-living and sessile forms of P. aeruginosa, as a possible mechanism for biofilm disruption. Furthermore, the findings that esculentin(1-21) is able to prolong survival of animals in models of sepsis and pulmonary infection indicate that this peptide can be a promising template for the generation of new antibiotic formulations to advance care of infections caused by P. aeruginosa

Esculentin(1-21), an amphibian skin membrane-active peptide with potent activity on both planktonic and biofilm cells of the bacterial pathogen Pseudomonas aeruginosa / Luca, Vincenzo; Stringaro, A; Colone, M; Pini, A; Mangoni, Maria Luisa. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - STAMPA. - 70:(2013), pp. 2773-2786. [10.1007/s00018-013-1291-7]

Esculentin(1-21), an amphibian skin membrane-active peptide with potent activity on both planktonic and biofilm cells of the bacterial pathogen Pseudomonas aeruginosa

LUCA, VINCENZO;MANGONI, Maria Luisa
2013

Abstract

Pseudomonas aeruginosa is an opportunistic bacterial pathogen that forms sessile communities, named biofilms. The non-motile forms are very difficult to eradicate and are often associated with the establishment of persistent infections, especially in patients with cystic fibrosis. The resistance of P. aeruginosa to conventional antibiotics has become a growing health concern worldwide and has prompted the search for new anti-infective agents with new modes of action. Naturally occurring antimicrobial peptides (AMPs) represent promising future template candidates. Here we report on the potent activity and membrane-perturbing effects of the amphibian AMP esculentin(1-21), on both the free-living and sessile forms of P. aeruginosa, as a possible mechanism for biofilm disruption. Furthermore, the findings that esculentin(1-21) is able to prolong survival of animals in models of sepsis and pulmonary infection indicate that this peptide can be a promising template for the generation of new antibiotic formulations to advance care of infections caused by P. aeruginosa
Anti-biofilm activity; Anti-Pseudomonas activity; Antimicrobial peptide; Cystic fibrosis; Membrane-permeation; Scanning electron microscopy
01 Pubblicazione su rivista::01a Articolo in rivista
Esculentin(1-21), an amphibian skin membrane-active peptide with potent activity on both planktonic and biofilm cells of the bacterial pathogen Pseudomonas aeruginosa / Luca, Vincenzo; Stringaro, A; Colone, M; Pini, A; Mangoni, Maria Luisa. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - STAMPA. - 70:(2013), pp. 2773-2786. [10.1007/s00018-013-1291-7]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/513512
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