Gene profile and functional changes upon IgD cross-linking were evaluated in chronic lymphocytic leukemia (CLL). Microarrays highlighted responsiveness to IgD in all cases, independently of clinico-biological characteristics. Stimulated samples exhibited the down-regulation of transcripts of B-cell receptor signaling and cell-adhesion at 24h and the up-modulation of differentiation and apoptosis genes at 48h. A significant increase in apoptosis upon ligation was also documented. Furthermore, comparison between IgD and IgM stimulation displayed a differential transcriptional/functional response. In conclusion, CLL respond to IgD displaying expression changes and cell-death enhancement, indicating the apoptosis induction via-IgD as an alternative approach for CLL management. © 2013 Elsevier Ltd.
IgD cross-linking induces gene expression profiling changes and enhances apoptosis in chronic lymphocytic leukemia cells / Tavolaro, S., Peragine, N., Chiaretti, S., Ricciardi, M.R., Raponi, S., Messina, M., Simona, S., Marinelli, M., DI MAIO, V., Mauro, F.R., DEL GIUDICE, I., Foa, R., Guarini, A.. - In: LEUKEMIA RESEARCH. - ISSN 0145-2126. - 37:4(2013), pp. 455-462. [10.1016/j.leukres.2012.12.019]
IgD cross-linking induces gene expression profiling changes and enhances apoptosis in chronic lymphocytic leukemia cells
TAVOLARO, SIMONA;PERAGINE, NADIA;CHIARETTI, sabina;RICCIARDI, Maria Rosaria;RAPONI, SARA;MESSINA, MONICA;MARINELLI, MARILISA;Valeria Di Maio;MAURO, Francesca Romana;DEL GIUDICE, ILARIA;FOA, Roberto;GUARINI, Anna
2013
Abstract
Gene profile and functional changes upon IgD cross-linking were evaluated in chronic lymphocytic leukemia (CLL). Microarrays highlighted responsiveness to IgD in all cases, independently of clinico-biological characteristics. Stimulated samples exhibited the down-regulation of transcripts of B-cell receptor signaling and cell-adhesion at 24h and the up-modulation of differentiation and apoptosis genes at 48h. A significant increase in apoptosis upon ligation was also documented. Furthermore, comparison between IgD and IgM stimulation displayed a differential transcriptional/functional response. In conclusion, CLL respond to IgD displaying expression changes and cell-death enhancement, indicating the apoptosis induction via-IgD as an alternative approach for CLL management. © 2013 Elsevier Ltd.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
