A new type of p-Benzoquinone activated support (agarose, polyvinyl alcohol, chitosan) which can be stored for more than one year, is described. Coupling yields of 10-95% were obtained for various compounds (propylamine, serum albumin, alpha-amylase). A retained activity of 150% for the immobilized laccase, was obtained. This peculiar result is probably due to the presence of hydroquinone groups on the support, which, as a substrate of laccase, can generate a simultancous affinity retention of the enzyme. © 1989 Science & Technology Letters.
Ready to use p-Benzoquinone activated supports for biochemical coupling, with special applications for laccase immobilization / Mircea Alexandru, Mateescu; Grazyna, Weltrowska; Agostinelli, Enzo; Richard Saint, Andre; Marek, Weltrowski; Mondovi', Bruno. - In: BIOTECHNOLOGY TECHNIQUES. - ISSN 0951-208X. - 3:6(1989), pp. 415-420. [10.1007/bf01875011]
Ready to use p-Benzoquinone activated supports for biochemical coupling, with special applications for laccase immobilization
AGOSTINELLI, Enzo;MONDOVI', Bruno
1989
Abstract
A new type of p-Benzoquinone activated support (agarose, polyvinyl alcohol, chitosan) which can be stored for more than one year, is described. Coupling yields of 10-95% were obtained for various compounds (propylamine, serum albumin, alpha-amylase). A retained activity of 150% for the immobilized laccase, was obtained. This peculiar result is probably due to the presence of hydroquinone groups on the support, which, as a substrate of laccase, can generate a simultancous affinity retention of the enzyme. © 1989 Science & Technology Letters.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
