Endocannabinoids (ECs) are endogenous compounds that interact with type-1 and type-2 cannabinoid receptors (CB 1 and CB 2), as well as non-cannabinoid receptors. The multitude of roles attributed to ECs makes them an emerging target of pharmacotherapy for a number of disparate diseases. Here a high-throughput bioanalytical method based on micro SPE (μ-SPE) followed by LC-MS/MS analysis for the simultaneous determination of the two major endocannabinoids 2-arachidonoylglycerol (2-AG) and N-arachidonoylethanolamine (anandamide, AEA) in human plasma is presented. The chromatographic conditions obtained with the fused-core column allowed a good separation in 10 min also of the AG isomers. A very simple and reliable extraction has been optimised by means of C18-modified tips: it requires only 100 μL of plasma and allows the use of minimal volumes of organic solvent. The present method allows a rapid and effective clean-up, which also minimises the isomerisation of 2-AG. The whole procedure has been validated following the FDA guidelines for bioanalytical methods validation: the satisfactory recovery values, the negligible matrix effect and the good values of accuracy and reproducibility make it a simple and high-throughput analytical tool for clinical and biochemical studies on endocannabinoid signaling in humans.

Determination of the two major endocannabinoids in human plasma by μ-SPE followed by HPLC-MS/MS / M., Sergi; N., Battista; Montesano, Camilla; Curini, Roberta; M., Maccarrone; D., Compagnone. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - ELETTRONICO. - 405:(2013), pp. 785-793. [10.1007/s00216-012-6273-3]

Determination of the two major endocannabinoids in human plasma by μ-SPE followed by HPLC-MS/MS

MONTESANO, CAMILLA;CURINI, Roberta;
2013

Abstract

Endocannabinoids (ECs) are endogenous compounds that interact with type-1 and type-2 cannabinoid receptors (CB 1 and CB 2), as well as non-cannabinoid receptors. The multitude of roles attributed to ECs makes them an emerging target of pharmacotherapy for a number of disparate diseases. Here a high-throughput bioanalytical method based on micro SPE (μ-SPE) followed by LC-MS/MS analysis for the simultaneous determination of the two major endocannabinoids 2-arachidonoylglycerol (2-AG) and N-arachidonoylethanolamine (anandamide, AEA) in human plasma is presented. The chromatographic conditions obtained with the fused-core column allowed a good separation in 10 min also of the AG isomers. A very simple and reliable extraction has been optimised by means of C18-modified tips: it requires only 100 μL of plasma and allows the use of minimal volumes of organic solvent. The present method allows a rapid and effective clean-up, which also minimises the isomerisation of 2-AG. The whole procedure has been validated following the FDA guidelines for bioanalytical methods validation: the satisfactory recovery values, the negligible matrix effect and the good values of accuracy and reproducibility make it a simple and high-throughput analytical tool for clinical and biochemical studies on endocannabinoid signaling in humans.
2013
2-Arachidonoylglycerol (2-AG), Arachidonoylethanolamine (anandamide; AEA), Endocannabinoids, LC-MS/MS, Plasma
01 Pubblicazione su rivista::01a Articolo in rivista
Determination of the two major endocannabinoids in human plasma by μ-SPE followed by HPLC-MS/MS / M., Sergi; N., Battista; Montesano, Camilla; Curini, Roberta; M., Maccarrone; D., Compagnone. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - ELETTRONICO. - 405:(2013), pp. 785-793. [10.1007/s00216-012-6273-3]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/508330
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