A major limitation in the development of crystallization conditions for integral membrane proteins is the lack of knowledge of the role surfactant plays in the interactions of these proteins. Previous experimental observations have brought attention to the features of surfactant solutions near the liquid-liquid phase boundary in the search for a rational mechanism that would guide and thus accelerate the screening of crystallization conditions. Towards this goal, we have developed mixtures of nonionic surfactants β-octyl glucoside (C8βG1) and octyl triethylene glycol (C8E3) with tunable microstructure and studied their role in crystallization of bacteriorhodopsin. Small-angle neutron scattering was used to determine the micellar properties of several mixtures near the cloud point curve. Crystallization experiments were carried out in these solutions and the kinetics of growth of the protein-surfactant complexes were followed by light scattering. Results indicate that the rate of growth and morphology of the aggregates depend on the proximity of the solution conditions to the cloud point curve, which can be tuned by varying the ratio of the two surfactants.

Phase behavior and structure of C8bG1/C8E3 surfactant solutions and their role in membrane protein crystallization / Santonicola, Mariagabriella; E. W., Kaler; A. M., Lenhoff. - STAMPA. - (2004). (Intervento presentato al convegno Annual Meeting of the American Crystallographic Association 2004 tenutosi a Chicago (IL), USA nel July 17–22, 2004).

Phase behavior and structure of C8bG1/C8E3 surfactant solutions and their role in membrane protein crystallization

SANTONICOLA, MARIAGABRIELLA;
2004

Abstract

A major limitation in the development of crystallization conditions for integral membrane proteins is the lack of knowledge of the role surfactant plays in the interactions of these proteins. Previous experimental observations have brought attention to the features of surfactant solutions near the liquid-liquid phase boundary in the search for a rational mechanism that would guide and thus accelerate the screening of crystallization conditions. Towards this goal, we have developed mixtures of nonionic surfactants β-octyl glucoside (C8βG1) and octyl triethylene glycol (C8E3) with tunable microstructure and studied their role in crystallization of bacteriorhodopsin. Small-angle neutron scattering was used to determine the micellar properties of several mixtures near the cloud point curve. Crystallization experiments were carried out in these solutions and the kinetics of growth of the protein-surfactant complexes were followed by light scattering. Results indicate that the rate of growth and morphology of the aggregates depend on the proximity of the solution conditions to the cloud point curve, which can be tuned by varying the ratio of the two surfactants.
2004
Annual Meeting of the American Crystallographic Association 2004
Crystallization kinetics; Surfactant mixtures; Scattering; Materials phase behavior; Protein-surfactant complexes
04 Pubblicazione in atti di convegno::04b Atto di convegno in volume
Phase behavior and structure of C8bG1/C8E3 surfactant solutions and their role in membrane protein crystallization / Santonicola, Mariagabriella; E. W., Kaler; A. M., Lenhoff. - STAMPA. - (2004). (Intervento presentato al convegno Annual Meeting of the American Crystallographic Association 2004 tenutosi a Chicago (IL), USA nel July 17–22, 2004).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/506309
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