The phase behavior of aqueous nonionic surfactant mixtures of n-octyl-b-D-glucopyranoside and triethylene glycol monooctyl ether (C8bG1 and C8E3) has been studied under conditions relevant to membrane protein crystallization. C8bG1 is widely used for crystallization of membrane proteins, and forms an isotropic liquid phase in aqueous solution at all temperatures up to 60% wt. We explored the effect of the cosurfactant C8E3 on the solubility of C8bG1. Structure and interactions of C8bG1-C8E3 mixed micelles have been investigated using small-angle neutron scattering in a buffer solution which is suitable for OmpF crystallization. Specifically, the solution behavior has been investigated as the solution conditions approach the phase boundary of the mixed surfactant system. Data from neutron scattering have been analyzed using a generalized indirect Fourier transformation procedure developed for the evaluation of scattering data of interacting particles. Results confirm previous evidence of the role of the phase boundary on micelle interactions and show how such phase behavior features could influence protein interactions.
Structure and interactions in nonionic surfactant solutions: implications for membrane protein crystallization / Santonicola, Mariagabriella; A. M., Lenhoff; E. W., Kaler. - (2003). (Intervento presentato al convegno 77th ACS Colloid and Surface Science Symposium tenutosi a Atlanta (GA), USA nel June 15–18, 2003).
Structure and interactions in nonionic surfactant solutions: implications for membrane protein crystallization
SANTONICOLA, MARIAGABRIELLA;
2003
Abstract
The phase behavior of aqueous nonionic surfactant mixtures of n-octyl-b-D-glucopyranoside and triethylene glycol monooctyl ether (C8bG1 and C8E3) has been studied under conditions relevant to membrane protein crystallization. C8bG1 is widely used for crystallization of membrane proteins, and forms an isotropic liquid phase in aqueous solution at all temperatures up to 60% wt. We explored the effect of the cosurfactant C8E3 on the solubility of C8bG1. Structure and interactions of C8bG1-C8E3 mixed micelles have been investigated using small-angle neutron scattering in a buffer solution which is suitable for OmpF crystallization. Specifically, the solution behavior has been investigated as the solution conditions approach the phase boundary of the mixed surfactant system. Data from neutron scattering have been analyzed using a generalized indirect Fourier transformation procedure developed for the evaluation of scattering data of interacting particles. Results confirm previous evidence of the role of the phase boundary on micelle interactions and show how such phase behavior features could influence protein interactions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
