Macromolecular nanotechnology can be used to develop switchable surfaces, especially so called polymer brushes are suitable for this. Stimulus-responsive polymers grafted from surfaces can be applied for example in biosensors and protein assays. The development of a biosensor for high throughput screening of membrane protein is a major challenge in drug development. Here we present different polymer brush structures, which can be used in such a membrane protein assay. Our aim is to chemically functionalize nanoporous supports so they can be used as platforms for membrane protein assays. For this we use polymer brushes grafted from surfaces using controlled polymerization techniques, most notably surface-initiated atom transfer radical polymerization (SI-ATRP). These polymer brushes provide robust and reproducible platforms with precise control of surface properties. pH-responsive poly(methacrylic acid) (PMAA) brushes were grafted from nanopore wall surfaces for controlled nanopore sensor function. Opening and closing of the pores were observed in situ by atomic force microscopy (AFM) in liquid environment upon varying the pH of the buffer solution Besides controlled opening and closing of the pores it is also possible to functionalize the carboxylic acid groups of PMAA with NTA, which can be used as a target for labeled membrane protein. So a membrane protein can be positioned over a nanopore. This research is part of the EU-FP7 project ASMENA “Functional Assays for Membrane Protein on Nanostructured Supports”.

Smart polymer brush structure for a high throughput membrane protein drug screening assay / G. W., DE GROOT; Santonicola, Mariagabriella; G. J., Vancso. - (2011). (Intervento presentato al convegno Netherlands MicroNano Conference '11 tenutosi a Ede, The Netherlands nel November 15-16, 2011).

Smart polymer brush structure for a high throughput membrane protein drug screening assay

SANTONICOLA, MARIAGABRIELLA;
2011

Abstract

Macromolecular nanotechnology can be used to develop switchable surfaces, especially so called polymer brushes are suitable for this. Stimulus-responsive polymers grafted from surfaces can be applied for example in biosensors and protein assays. The development of a biosensor for high throughput screening of membrane protein is a major challenge in drug development. Here we present different polymer brush structures, which can be used in such a membrane protein assay. Our aim is to chemically functionalize nanoporous supports so they can be used as platforms for membrane protein assays. For this we use polymer brushes grafted from surfaces using controlled polymerization techniques, most notably surface-initiated atom transfer radical polymerization (SI-ATRP). These polymer brushes provide robust and reproducible platforms with precise control of surface properties. pH-responsive poly(methacrylic acid) (PMAA) brushes were grafted from nanopore wall surfaces for controlled nanopore sensor function. Opening and closing of the pores were observed in situ by atomic force microscopy (AFM) in liquid environment upon varying the pH of the buffer solution Besides controlled opening and closing of the pores it is also possible to functionalize the carboxylic acid groups of PMAA with NTA, which can be used as a target for labeled membrane protein. So a membrane protein can be positioned over a nanopore. This research is part of the EU-FP7 project ASMENA “Functional Assays for Membrane Protein on Nanostructured Supports”.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/506290
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