cDNA encoding human 4-aminobutyrate aminotransferase (aminobutyrate:2-oxoglutarate aminotransferase) was prepared by polymerase chain reaction using mRNA from human neuroblastoma cells as the template and oligonucleotides synthesized on the basis of the information obtained from direct protein sequencing. The cDNA-deduced sequence enabled peptides, sequenced by automated Edman degradation, to be aligned for confirmation of the complete primary structure. The results are compared with the recently published sequence of the rat enzyme deduced entirely from DNA sequencing [Medina-Kauwe, L. K., Tillakaratne, N. J. K., Wu, J.-Y. and Tobin, A. J. (1994) J. Neurochem. 62, 1267-1275]. Although the sequences are almost identical for most of their length, they differ in a segment of 36 residues. Almost complete identity of the two sequences is established if it is assumed that a frame-shift error was introduced into the reported rat cDNA sequence. The human cDNA was used to probe for the presence of 4-aminobutyrate aminotransferase mRNA in human tissues and a significant transcript was found in heart, placenta and in tissues usually associated with the expression of this enzyme.

PRIMARY STRUCTURE AND TISSUE DISTRIBUTION OF HUMAN 4-AMINOBUTYRATE AMINOTRANSFERASE / DE BIASE, Daniela; Barra, Donatella; Simmaco, Maurizio; John, Ra; Bossa, Francesco. - In: EUROPEAN JOURNAL OF BIOCHEMISTRY. - ISSN 0014-2956. - STAMPA. - 227:1-2(1995), pp. 476-480. [10.1111/j.1432-1033.1995.tb20412.x]

PRIMARY STRUCTURE AND TISSUE DISTRIBUTION OF HUMAN 4-AMINOBUTYRATE AMINOTRANSFERASE

DE BIASE, Daniela;BARRA, Donatella;SIMMACO, Maurizio;BOSSA, Francesco
1995

Abstract

cDNA encoding human 4-aminobutyrate aminotransferase (aminobutyrate:2-oxoglutarate aminotransferase) was prepared by polymerase chain reaction using mRNA from human neuroblastoma cells as the template and oligonucleotides synthesized on the basis of the information obtained from direct protein sequencing. The cDNA-deduced sequence enabled peptides, sequenced by automated Edman degradation, to be aligned for confirmation of the complete primary structure. The results are compared with the recently published sequence of the rat enzyme deduced entirely from DNA sequencing [Medina-Kauwe, L. K., Tillakaratne, N. J. K., Wu, J.-Y. and Tobin, A. J. (1994) J. Neurochem. 62, 1267-1275]. Although the sequences are almost identical for most of their length, they differ in a segment of 36 residues. Almost complete identity of the two sequences is established if it is assumed that a frame-shift error was introduced into the reported rat cDNA sequence. The human cDNA was used to probe for the presence of 4-aminobutyrate aminotransferase mRNA in human tissues and a significant transcript was found in heart, placenta and in tissues usually associated with the expression of this enzyme.
1995
4-aminobutyrate; 4-aminobutyrate aminotransferase; gaba; primary structure; pyridoxal 5'-phosphate-dependent enzyme; pyridoxal-phosphate-dependent enzyme; tissue distribution
01 Pubblicazione su rivista::01a Articolo in rivista
PRIMARY STRUCTURE AND TISSUE DISTRIBUTION OF HUMAN 4-AMINOBUTYRATE AMINOTRANSFERASE / DE BIASE, Daniela; Barra, Donatella; Simmaco, Maurizio; John, Ra; Bossa, Francesco. - In: EUROPEAN JOURNAL OF BIOCHEMISTRY. - ISSN 0014-2956. - STAMPA. - 227:1-2(1995), pp. 476-480. [10.1111/j.1432-1033.1995.tb20412.x]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/50380
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 26
social impact