White blood cells are isolated from whole blood in silicon–glass 4.5-ml microchips containing a series of 3.5-mm feature-sized ‘weir-type‘ filters, formed by an etched silicon dam spanning the flow chamber. Genomic DNA targets, e.g., dystrophin gene, can be directly amplified using the polymerase chain reaction (PCR) from the white cells isolated on the filters. This dual function microchip provides a means to simplify nucleic acid analyses by integrating in a single device two key steps in the analytical procedure, namely, cell isolation and PCR
Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers / Peter, Wilding; Larry J., Kricka; Jing, Cheng; Gia, Hvichia; Mann A., Shoffner; Fortina, Paolo. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - STAMPA. - 257:2(1998), pp. 95-100. [10.1006/abio.1997.2530]
Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers.
FORTINA, PAOLO
1998
Abstract
White blood cells are isolated from whole blood in silicon–glass 4.5-ml microchips containing a series of 3.5-mm feature-sized ‘weir-type‘ filters, formed by an etched silicon dam spanning the flow chamber. Genomic DNA targets, e.g., dystrophin gene, can be directly amplified using the polymerase chain reaction (PCR) from the white cells isolated on the filters. This dual function microchip provides a means to simplify nucleic acid analyses by integrating in a single device two key steps in the analytical procedure, namely, cell isolation and PCRI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
