The chemical nature of Sindbis virus receptors on goose erythrocytes was investigated by means of two different experimental approaches: by testing the capacity of human serum lipoproteins (HDL, LDL, VLDL) to compete with erythrocytes for virus binding and by enzymatic and chemical modifications of the cell membrane. Each class of lipoproteins was separated into its protein and lipid components which were tested for hemagglutination and hemolysis inhibiting activity. Only the lipid moieties were able to inhibit binding and fusion activities of the virus. Enzymatic treatments of the erythrocyte surface showed that sialic acid and trypsin-sensitive proteins were not involved in Sindbis virus membrane receptor and that some phospholipids probably represent important components of the cellular binding site.

involvement of lipids in the interaction of sindbis virus with goose erythrocytes / Mastromarino, Paola; Conti, Cinzia; Rieti, S.; Orsi, Nicola. - In: MICROBIOLOGICA. - ISSN 0391-5352. - STAMPA. - 12:(1990), pp. 113-120.

involvement of lipids in the interaction of sindbis virus with goose erythrocytes

MASTROMARINO, Paola;CONTI, Cinzia;ORSI, Nicola
1990

Abstract

The chemical nature of Sindbis virus receptors on goose erythrocytes was investigated by means of two different experimental approaches: by testing the capacity of human serum lipoproteins (HDL, LDL, VLDL) to compete with erythrocytes for virus binding and by enzymatic and chemical modifications of the cell membrane. Each class of lipoproteins was separated into its protein and lipid components which were tested for hemagglutination and hemolysis inhibiting activity. Only the lipid moieties were able to inhibit binding and fusion activities of the virus. Enzymatic treatments of the erythrocyte surface showed that sialic acid and trypsin-sensitive proteins were not involved in Sindbis virus membrane receptor and that some phospholipids probably represent important components of the cellular binding site.
1990
01 Pubblicazione su rivista::01a Articolo in rivista
involvement of lipids in the interaction of sindbis virus with goose erythrocytes / Mastromarino, Paola; Conti, Cinzia; Rieti, S.; Orsi, Nicola. - In: MICROBIOLOGICA. - ISSN 0391-5352. - STAMPA. - 12:(1990), pp. 113-120.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/502567
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