The aerobic metabolism of a mixture of acetate and starch was studied with main emphasis on their interaction and the effect on their storage as PHB and glycogen, respectively. Pulse feeding strongly increased the storage of both substrates; however, the presence of starch decreased PHB storage whereas the presence of acetate did not affect glycogen storage. Indeed, C-13 NMR isotopomer analysis suggested an increase of acetate utilization towards TCA cycle, due to an increased request of ATP production for glycogen biosynthesis regulated by ADP-GlcPPase. This in turn influenced the partition flux for pyruvate synthesis between TCA cataplerosis and glyoxylate shunt. The corresponding reduction of PHB synthesis was in agreement with the competition for HS-CoA between KGDH activity and acetyl-CoA for PHB synthesis pathway. As a practical consequence, bioprocesses for PHA production from volatile fatty acids could be negatively affected from other carbon sources, such as unfermented carbohydrates. (C) 2012 Elsevier Ltd. All rights reserved.

Aerobic metabolism of mixed carbon sources in sequencing batch reactor under pulse and continuous feeding / Asli S., Ciggin; Derin, Orhon; Donatella, Capitani; Miccheli, Alfredo; Puccetti, Caterina; Majone, Mauro. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 129:(2013), pp. 118-126. [10.1016/j.biortech.2012.10.109]

Aerobic metabolism of mixed carbon sources in sequencing batch reactor under pulse and continuous feeding

MICCHELI, Alfredo;PUCCETTI, Caterina;MAJONE, Mauro
2013

Abstract

The aerobic metabolism of a mixture of acetate and starch was studied with main emphasis on their interaction and the effect on their storage as PHB and glycogen, respectively. Pulse feeding strongly increased the storage of both substrates; however, the presence of starch decreased PHB storage whereas the presence of acetate did not affect glycogen storage. Indeed, C-13 NMR isotopomer analysis suggested an increase of acetate utilization towards TCA cycle, due to an increased request of ATP production for glycogen biosynthesis regulated by ADP-GlcPPase. This in turn influenced the partition flux for pyruvate synthesis between TCA cataplerosis and glyoxylate shunt. The corresponding reduction of PHB synthesis was in agreement with the competition for HS-CoA between KGDH activity and acetyl-CoA for PHB synthesis pathway. As a practical consequence, bioprocesses for PHA production from volatile fatty acids could be negatively affected from other carbon sources, such as unfermented carbohydrates. (C) 2012 Elsevier Ltd. All rights reserved.
13c nmr; 13c-nmr; activated sludge; c-13 nmr; glycogen; isotopomer analysis; phb
01 Pubblicazione su rivista::01a Articolo in rivista
Aerobic metabolism of mixed carbon sources in sequencing batch reactor under pulse and continuous feeding / Asli S., Ciggin; Derin, Orhon; Donatella, Capitani; Miccheli, Alfredo; Puccetti, Caterina; Majone, Mauro. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 129:(2013), pp. 118-126. [10.1016/j.biortech.2012.10.109]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/500839
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