Junctional transfer of ions in monolayer primary cultures of Sertoli cells and in intact seminiferous tubules from 20 day-old rats has been investigated by using electrophysiological techniques. The electrotonic coupling of both intact tubule and monolayer culture was inhibited by the presence of dibutyryl cyclic AMP (10-4 M) in the medium. In contrast, 1-oleoy1-2-acetylglycerol (10-5 M) and 12-0-tetradecanoyl-phorbol-13-acetate (10-7 M) decreased the junctional resistivity and increased the extent of the coupling in immature tubules. The significance of this modulation of the cell coupling in the seminiferous epithelium is discussed. © 1986.
Putative second messengers affect cell coupling in the seminiferous tubules / Grassi, Francesca; Monaco, Lucia; G., Fratamico; S., Dolci; E., Iannini; M., Conti; Eusebi, Fabrizio; Stefanini, Mario. - In: CELL BIOLOGY INTERNATIONAL REPORTS. - ISSN 0309-1651. - STAMPA. - 10:8(1986), pp. 631-639. [10.1016/0309-1651(86)90140-2]
Putative second messengers affect cell coupling in the seminiferous tubules
GRASSI, Francesca;MONACO, Lucia;EUSEBI, Fabrizio;STEFANINI, Mario
1986
Abstract
Junctional transfer of ions in monolayer primary cultures of Sertoli cells and in intact seminiferous tubules from 20 day-old rats has been investigated by using electrophysiological techniques. The electrotonic coupling of both intact tubule and monolayer culture was inhibited by the presence of dibutyryl cyclic AMP (10-4 M) in the medium. In contrast, 1-oleoy1-2-acetylglycerol (10-5 M) and 12-0-tetradecanoyl-phorbol-13-acetate (10-7 M) decreased the junctional resistivity and increased the extent of the coupling in immature tubules. The significance of this modulation of the cell coupling in the seminiferous epithelium is discussed. © 1986.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
