Dielectric measurements on different ribosome suspensions were carried out in the frequency range from 10 kHz to 1 GHz. In intact ribosomes two dispersions were detected: one around 100 kHz and the other one in the MHz region. In separated ribosomal subunits and in ribosomes resuspended in a buffer with no magnesium ions (relaxed ribosomes) only the MHz dispersion was observed. Electrical conductivities of the samples at 1 kHz were also measured. The temperature dependence of the two dispersions was investigated and a tentative attribution was proposed.
Radiofrequency dielectric spectroscopy of ribosome suspensions / Bonincontro, Adalberto; Giansanti, Andrea; F., Pedone; G., Risuleo. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - STAMPA. - 1115:(1991), pp. 49-53. [10.1016/0304-4165(91)90010-E]
Radiofrequency dielectric spectroscopy of ribosome suspensions
BONINCONTRO, Adalberto;GIANSANTI, Andrea;
1991
Abstract
Dielectric measurements on different ribosome suspensions were carried out in the frequency range from 10 kHz to 1 GHz. In intact ribosomes two dispersions were detected: one around 100 kHz and the other one in the MHz region. In separated ribosomal subunits and in ribosomes resuspended in a buffer with no magnesium ions (relaxed ribosomes) only the MHz dispersion was observed. Electrical conductivities of the samples at 1 kHz were also measured. The temperature dependence of the two dispersions was investigated and a tentative attribution was proposed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
