Electrical conductivity and dielectric dispersions have been measured on suspensions of 30S, 50S subunits and 70S ribosomal particles from E. coli, in the presence and absence of magnesium ions. Conductivity measurements show a net contribution due to the disperzed phase. Ribosomal particles act in general as obstructants to the ionic motions in the solution, but besides this effect, there is an intrinsic contribution to the overall conductivity which is more pronounced in the absence than in the presence of magnesium ions. This effect suggests the existence of an interfacial conductivity, modulated by bound magnesium ions. The intrinsic conductivity is higher in the 30S subunit than in the 50S. Dielectric dispersions show a consistent difference between 30S and 50S, indicating that the exposure of ribosomal RNA should be greater in the 30S than in the 50S. Structural information is derived from the conductivity measurement, via the Looyenga equation.

Electrical conductivity and dielectric dispersion of E. coli 70S ribosomes and of 30S and 50S subunits: effects of magnesium ions / Bonincontro, Adalberto; Briganti, Giuseppe; Giansanti, Andrea; F., Pedone; G., Risuleo. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - STAMPA. - 6:(1996), pp. 219-226. [10.1016/0927-7765(95)01254-0]

Electrical conductivity and dielectric dispersion of E. coli 70S ribosomes and of 30S and 50S subunits: effects of magnesium ions

BONINCONTRO, Adalberto;BRIGANTI, Giuseppe;GIANSANTI, Andrea;
1996

Abstract

Electrical conductivity and dielectric dispersions have been measured on suspensions of 30S, 50S subunits and 70S ribosomal particles from E. coli, in the presence and absence of magnesium ions. Conductivity measurements show a net contribution due to the disperzed phase. Ribosomal particles act in general as obstructants to the ionic motions in the solution, but besides this effect, there is an intrinsic contribution to the overall conductivity which is more pronounced in the absence than in the presence of magnesium ions. This effect suggests the existence of an interfacial conductivity, modulated by bound magnesium ions. The intrinsic conductivity is higher in the 30S subunit than in the 50S. Dielectric dispersions show a consistent difference between 30S and 50S, indicating that the exposure of ribosomal RNA should be greater in the 30S than in the 50S. Structural information is derived from the conductivity measurement, via the Looyenga equation.
1996
01 Pubblicazione su rivista::01a Articolo in rivista
Electrical conductivity and dielectric dispersion of E. coli 70S ribosomes and of 30S and 50S subunits: effects of magnesium ions / Bonincontro, Adalberto; Briganti, Giuseppe; Giansanti, Andrea; F., Pedone; G., Risuleo. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - STAMPA. - 6:(1996), pp. 219-226. [10.1016/0927-7765(95)01254-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/497061
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