The peptide fraction extracted by methanol from the skin of Rana esculenta, a species widely distributed in Western Europe, was investigated. The pharmacological activity found in the extract is attributable to the presence of authentic bradykinin, together with a shorter, partially active version of this molecule, des-Arg9-bradykinin. Also the bradykinin fragment 1-7 has been isolated, but it was inactive in our bioassay system. Moreover, a family of hydrophobic peptides has been purified and characterized, which appeared devoid of pharmacological activities when tested on smooth muscle preparations, but were provided with hemolytic activities.
Purification and characterization of bioactive peptides from the skin extract of Rana esculenta / Simmaco, Maurizio; DE BIASE, Daniela; Severini, C; Aita, M; FALCONIERI ERSPAMER, G; Barra, Donatella; Bossa, Francesco. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1033:(1990), pp. 318-323. [10.1016/0304-4165(90)90140-R]
Purification and characterization of bioactive peptides from the skin extract of Rana esculenta.
SIMMACO, Maurizio;DE BIASE, Daniela;BARRA, Donatella;BOSSA, Francesco
1990
Abstract
The peptide fraction extracted by methanol from the skin of Rana esculenta, a species widely distributed in Western Europe, was investigated. The pharmacological activity found in the extract is attributable to the presence of authentic bradykinin, together with a shorter, partially active version of this molecule, des-Arg9-bradykinin. Also the bradykinin fragment 1-7 has been isolated, but it was inactive in our bioassay system. Moreover, a family of hydrophobic peptides has been purified and characterized, which appeared devoid of pharmacological activities when tested on smooth muscle preparations, but were provided with hemolytic activities.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
