High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99mTc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability
Efficient one-step direct labelling of recombinant antibodies with technetium-99m / Liberatore, Mauro; D., Neri; G., Neri; A., Pini; A. P., Iurilli; F., Ponzo; G., Spampinato; F., Padula; Pala, Alessandro; A. C., Colella. - In: EUROPEAN JOURNAL OF NUCLEAR MEDICINE. - ISSN 0340-6997. - STAMPA. - 22:11(1995), pp. 1326-1329. [10.1007/bf00801622]
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Titolo: | Efficient one-step direct labelling of recombinant antibodies with technetium-99m | |
Autori: | ||
Data di pubblicazione: | 1995 | |
Rivista: | ||
Citazione: | Efficient one-step direct labelling of recombinant antibodies with technetium-99m / Liberatore, Mauro; D., Neri; G., Neri; A., Pini; A. P., Iurilli; F., Ponzo; G., Spampinato; F., Padula; Pala, Alessandro; A. C., Colella. - In: EUROPEAN JOURNAL OF NUCLEAR MEDICINE. - ISSN 0340-6997. - STAMPA. - 22:11(1995), pp. 1326-1329. [10.1007/bf00801622] | |
Handle: | http://hdl.handle.net/11573/491843 | |
Appartiene alla tipologia: | 01a Articolo in rivista |