Two rapid and reliable electrophoretic techniques for PGM1 and EsD typing on ultrathin polyacrylamide gels are described. They have been based on non-equilibrium pH gradient electrophoresis and on the addition of chemical spacers (EPPS for PGM1 and HEPES for EsD) to the gel mixture.
Non-equilibrium pH gradient electrophoresis (nephge) on ultrathin polyacrylamide gels containing separators: Improved erythrocyte phosphoglucomutase (PGM1) and esterase D (EsD) diagnosis in red cell lysates and bloodstains / DESTRO-BISOL, Giovanni; Aldo, Spinella. - In: FORENSIC SCIENCE INTERNATIONAL. - ISSN 0379-0738. - STAMPA. - 42:1-2(1989), pp. 43-50. [10.1016/0379-0738(89)90197-7]
Non-equilibrium pH gradient electrophoresis (nephge) on ultrathin polyacrylamide gels containing separators: Improved erythrocyte phosphoglucomutase (PGM1) and esterase D (EsD) diagnosis in red cell lysates and bloodstains
DESTRO-BISOL, Giovanni;
1989
Abstract
Two rapid and reliable electrophoretic techniques for PGM1 and EsD typing on ultrathin polyacrylamide gels are described. They have been based on non-equilibrium pH gradient electrophoresis and on the addition of chemical spacers (EPPS for PGM1 and HEPES for EsD) to the gel mixture.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
