The recombinant plasmid pRI203 carries a Yersinia pseudotuberculosis chromosomal gene that makes E. coli K-12 HB101 strain able to synthetize an outer membrane protein, invasin, which interacts with integrin receptors of eukaryotic cells, enabling this microorganism to penetrate human cultured animal cells. In this study we evaluated the involvement of HeLa cell membrane structural components in the early phases of the invasive pathway of E. coli HB101 (pRI203). When HeLa cell monolayers were treated with several enzymes we showed that trypsin-, proteinase K- and neuraminidase-sensitive components are required for bacterial invasion. Comparison of the ability of simple and complex carbohydrates to inhibit bacterial invasion indicated that N-acetyl neuraminic acid, N-acetyl glucosamine and mucin were the most effective competitive inhibitors. Among glycolipids, gangliosides enhanced bacterial entry in HeLa cells. The results obtained suggest that N-acetyl neuraminic acid and N-acetyl glucosamine-containing glycoproteins and/or glycolipids participate as putative HeLa cell binding sites for the penetration process of E. coli HB101 (pRI203).

INVOLVEMENT OF MEMBRANE CARBOHYDRATES OF HELA-CELLS IN THE ESCHERICHIA-COLI HB101 (PRI203) INVASIVE PATHWAY / Longhi, Catia; Conte, Maria Pia; Nicoletti, M; Valenti, Piera; Seganti, Lucilla. - In: MICROBIOLOGICA. - ISSN 0391-5352. - STAMPA. - 15:(1992), pp. 107-115.

INVOLVEMENT OF MEMBRANE CARBOHYDRATES OF HELA-CELLS IN THE ESCHERICHIA-COLI HB101 (PRI203) INVASIVE PATHWAY

LONGHI, Catia;CONTE, Maria Pia;VALENTI, PIERA;SEGANTI, Lucilla
1992

Abstract

The recombinant plasmid pRI203 carries a Yersinia pseudotuberculosis chromosomal gene that makes E. coli K-12 HB101 strain able to synthetize an outer membrane protein, invasin, which interacts with integrin receptors of eukaryotic cells, enabling this microorganism to penetrate human cultured animal cells. In this study we evaluated the involvement of HeLa cell membrane structural components in the early phases of the invasive pathway of E. coli HB101 (pRI203). When HeLa cell monolayers were treated with several enzymes we showed that trypsin-, proteinase K- and neuraminidase-sensitive components are required for bacterial invasion. Comparison of the ability of simple and complex carbohydrates to inhibit bacterial invasion indicated that N-acetyl neuraminic acid, N-acetyl glucosamine and mucin were the most effective competitive inhibitors. Among glycolipids, gangliosides enhanced bacterial entry in HeLa cells. The results obtained suggest that N-acetyl neuraminic acid and N-acetyl glucosamine-containing glycoproteins and/or glycolipids participate as putative HeLa cell binding sites for the penetration process of E. coli HB101 (pRI203).
1992
01 Pubblicazione su rivista::01a Articolo in rivista
INVOLVEMENT OF MEMBRANE CARBOHYDRATES OF HELA-CELLS IN THE ESCHERICHIA-COLI HB101 (PRI203) INVASIVE PATHWAY / Longhi, Catia; Conte, Maria Pia; Nicoletti, M; Valenti, Piera; Seganti, Lucilla. - In: MICROBIOLOGICA. - ISSN 0391-5352. - STAMPA. - 15:(1992), pp. 107-115.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/486706
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