A QuEChERS (Quick Easy Cheap Effective Rugged Safe)-like extraction method was developed for the simultaneous analysis of veterinary drugs and mycotoxins in hen eggs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray (ESI) source. Various classes of antimicrobials (tetracyclines, ionophores, coccidiostats, penicillins, cephalosporins, fluoroquinolones, sulfonamides) and mycotoxins (enniatins, beauvericin, ochratoxins, aflatoxins) were considered for the development of this method. Particular attention was devoted to extraction optimization: different solvents (acetone, acetonitrile and methanol), different pH values and different sample to extracting volume ratios were tested and evaluated in terms of recovery, relative standard deviation (RSD) and ESI signal suppression due to matrix effect. Chromatographic and mass spectrometric conditions were optimized to obtain the best instrumental performances for most of the analytes. Quantitative analysis was performed by means of matrix-matched calibration, in a range that varied depending on the analyte and its established maximum limit, when there was one. Recoveries at 100 mu g kg(-1) spiking level were >62% (3 < RSD < 17) for all the antimicrobials except lasalocid A (41 +/- 10%) and oxacillin (56 +/- 17%). The multiclass method proposed is rapid, simple, and involves a low solvent consumption, in line with QuEChERS features and modern analytical requirements. (C) 2012 Elsevier B.V. All rights reserved.

Multiclass screening method based on solvent extraction and liquid chromatography-tandem mass spectrometry for the determination of antimicrobials and mycotoxins in egg / Capriotti, ANNA LAURA; Cavaliere, Chiara; Piovesana, Susy; Samperi, Roberto; Lagana', Aldo. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 1873-3778. - STAMPA. - 1268(2012), pp. 84-90. [10.1016/j.chroma.2012.10.040]

Multiclass screening method based on solvent extraction and liquid chromatography-tandem mass spectrometry for the determination of antimicrobials and mycotoxins in egg

CAPRIOTTI, ANNA LAURA;CAVALIERE, CHIARA;PIOVESANA, SUSY;SAMPERI, Roberto;LAGANA', Aldo
2012

Abstract

A QuEChERS (Quick Easy Cheap Effective Rugged Safe)-like extraction method was developed for the simultaneous analysis of veterinary drugs and mycotoxins in hen eggs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray (ESI) source. Various classes of antimicrobials (tetracyclines, ionophores, coccidiostats, penicillins, cephalosporins, fluoroquinolones, sulfonamides) and mycotoxins (enniatins, beauvericin, ochratoxins, aflatoxins) were considered for the development of this method. Particular attention was devoted to extraction optimization: different solvents (acetone, acetonitrile and methanol), different pH values and different sample to extracting volume ratios were tested and evaluated in terms of recovery, relative standard deviation (RSD) and ESI signal suppression due to matrix effect. Chromatographic and mass spectrometric conditions were optimized to obtain the best instrumental performances for most of the analytes. Quantitative analysis was performed by means of matrix-matched calibration, in a range that varied depending on the analyte and its established maximum limit, when there was one. Recoveries at 100 mu g kg(-1) spiking level were >62% (3 < RSD < 17) for all the antimicrobials except lasalocid A (41 +/- 10%) and oxacillin (56 +/- 17%). The multiclass method proposed is rapid, simple, and involves a low solvent consumption, in line with QuEChERS features and modern analytical requirements. (C) 2012 Elsevier B.V. All rights reserved.
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/485006
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 73
  • ???jsp.display-item.citation.isi??? 68
social impact